The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients

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The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients. / Otte, Julia M.; Harter, Johannes; Laufer, Katja; Blackwell, Nia; Straub, Daniel; Kappler, Andreas; Kleindienst, Sara.

In: Environmental Microbiology, Vol. 20, No. 7, 07.2018, p. 2483-2499.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Otte, JM, Harter, J, Laufer, K, Blackwell, N, Straub, D, Kappler, A & Kleindienst, S 2018, 'The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients', Environmental Microbiology, vol. 20, no. 7, pp. 2483-2499. https://doi.org/10.1111/1462-2920.14260

APA

Otte, J. M., Harter, J., Laufer, K., Blackwell, N., Straub, D., Kappler, A., & Kleindienst, S. (2018). The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients. Environmental Microbiology, 20(7), 2483-2499. https://doi.org/10.1111/1462-2920.14260

CBE

Otte JM, Harter J, Laufer K, Blackwell N, Straub D, Kappler A, Kleindienst S. 2018. The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients. Environmental Microbiology. 20(7):2483-2499. https://doi.org/10.1111/1462-2920.14260

MLA

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Author

Otte, Julia M. ; Harter, Johannes ; Laufer, Katja ; Blackwell, Nia ; Straub, Daniel ; Kappler, Andreas ; Kleindienst, Sara. / The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients. In: Environmental Microbiology. 2018 ; Vol. 20, No. 7. pp. 2483-2499.

Bibtex

@article{a90ba54b23814b349817efbb8dece673,
title = "The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients",
abstract = "Microaerophilic, phototrophic and nitrate-reducing Fe(II)-oxidizers co-exist in coastal marine and littoral freshwater sediments. However, the in situ abundance, distribution and diversity of metabolically active Fe(II)-oxidizers remained largely unexplored. Here, we characterized the microbial community composition at the oxic-anoxic interface of littoral freshwater (Lake Constance, Germany) and coastal marine sediments (Kalo Vig and Norsminde Fjord, Denmark) using DNA-/RNA-based next-generation 16S rRNA (gene) amplicon sequencing. All three physiological groups of neutrophilic Fe(II)-oxidizing bacteria were found to be active in marine and freshwater sediments, revealing up to 0.2% anoxygenic photoferrotrophs (e.g., Rhodopseudomonas, Rhodobacter, Chlorobium), 0.1% microaerophilic Fe(II)-oxidizers (e.g., Mariprofundus, Hyphomonas, Gallionella) and 0.3% nitrate-reducing Fe(II)-oxidizers (e.g., Thiobacillus, Pseudomonas, Denitromonas, Hoeflea). Active Fe(III)-reducing bacteria (e.g., Shewanella, Geobacter) were most abundant (up to 2.8%) in marine sediments and co-occurred with cable bacteria (up to 4.5%). Geochemical profiles of Fe(III), Fe(II), O-2, light, nitrate and total organic carbon revealed a redox stratification of the sediments and explained 75%-85% of the vertical distribution of microbial taxa, while active Fe-cycling bacteria were found to be decoupled from geochemical gradients. We suggest that metabolic flexibility, microniches in the sediments, or interrelationships with cable bacteria might explain the distribution patterns of active Fe-cycling bacteria.",
keywords = "SULFATE-REDUCING BACTERIA, PALUSTRIS STRAIN TIE-1, FERROUS-IRON, CABLE BACTERIA, COMMUNITY STRUCTURE, NITRATE REDUCTION, FE(II) OXIDATION, ORGANIC-MATTER, SEQUENCE DATA, GALLIONELLA-FERRUGINEA",
author = "Otte, {Julia M.} and Johannes Harter and Katja Laufer and Nia Blackwell and Daniel Straub and Andreas Kappler and Sara Kleindienst",
year = "2018",
month = jul,
doi = "10.1111/1462-2920.14260",
language = "English",
volume = "20",
pages = "2483--2499",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "7",

}

RIS

TY - JOUR

T1 - The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients

AU - Otte, Julia M.

AU - Harter, Johannes

AU - Laufer, Katja

AU - Blackwell, Nia

AU - Straub, Daniel

AU - Kappler, Andreas

AU - Kleindienst, Sara

PY - 2018/7

Y1 - 2018/7

N2 - Microaerophilic, phototrophic and nitrate-reducing Fe(II)-oxidizers co-exist in coastal marine and littoral freshwater sediments. However, the in situ abundance, distribution and diversity of metabolically active Fe(II)-oxidizers remained largely unexplored. Here, we characterized the microbial community composition at the oxic-anoxic interface of littoral freshwater (Lake Constance, Germany) and coastal marine sediments (Kalo Vig and Norsminde Fjord, Denmark) using DNA-/RNA-based next-generation 16S rRNA (gene) amplicon sequencing. All three physiological groups of neutrophilic Fe(II)-oxidizing bacteria were found to be active in marine and freshwater sediments, revealing up to 0.2% anoxygenic photoferrotrophs (e.g., Rhodopseudomonas, Rhodobacter, Chlorobium), 0.1% microaerophilic Fe(II)-oxidizers (e.g., Mariprofundus, Hyphomonas, Gallionella) and 0.3% nitrate-reducing Fe(II)-oxidizers (e.g., Thiobacillus, Pseudomonas, Denitromonas, Hoeflea). Active Fe(III)-reducing bacteria (e.g., Shewanella, Geobacter) were most abundant (up to 2.8%) in marine sediments and co-occurred with cable bacteria (up to 4.5%). Geochemical profiles of Fe(III), Fe(II), O-2, light, nitrate and total organic carbon revealed a redox stratification of the sediments and explained 75%-85% of the vertical distribution of microbial taxa, while active Fe-cycling bacteria were found to be decoupled from geochemical gradients. We suggest that metabolic flexibility, microniches in the sediments, or interrelationships with cable bacteria might explain the distribution patterns of active Fe-cycling bacteria.

AB - Microaerophilic, phototrophic and nitrate-reducing Fe(II)-oxidizers co-exist in coastal marine and littoral freshwater sediments. However, the in situ abundance, distribution and diversity of metabolically active Fe(II)-oxidizers remained largely unexplored. Here, we characterized the microbial community composition at the oxic-anoxic interface of littoral freshwater (Lake Constance, Germany) and coastal marine sediments (Kalo Vig and Norsminde Fjord, Denmark) using DNA-/RNA-based next-generation 16S rRNA (gene) amplicon sequencing. All three physiological groups of neutrophilic Fe(II)-oxidizing bacteria were found to be active in marine and freshwater sediments, revealing up to 0.2% anoxygenic photoferrotrophs (e.g., Rhodopseudomonas, Rhodobacter, Chlorobium), 0.1% microaerophilic Fe(II)-oxidizers (e.g., Mariprofundus, Hyphomonas, Gallionella) and 0.3% nitrate-reducing Fe(II)-oxidizers (e.g., Thiobacillus, Pseudomonas, Denitromonas, Hoeflea). Active Fe(III)-reducing bacteria (e.g., Shewanella, Geobacter) were most abundant (up to 2.8%) in marine sediments and co-occurred with cable bacteria (up to 4.5%). Geochemical profiles of Fe(III), Fe(II), O-2, light, nitrate and total organic carbon revealed a redox stratification of the sediments and explained 75%-85% of the vertical distribution of microbial taxa, while active Fe-cycling bacteria were found to be decoupled from geochemical gradients. We suggest that metabolic flexibility, microniches in the sediments, or interrelationships with cable bacteria might explain the distribution patterns of active Fe-cycling bacteria.

KW - SULFATE-REDUCING BACTERIA

KW - PALUSTRIS STRAIN TIE-1

KW - FERROUS-IRON

KW - CABLE BACTERIA

KW - COMMUNITY STRUCTURE

KW - NITRATE REDUCTION

KW - FE(II) OXIDATION

KW - ORGANIC-MATTER

KW - SEQUENCE DATA

KW - GALLIONELLA-FERRUGINEA

U2 - 10.1111/1462-2920.14260

DO - 10.1111/1462-2920.14260

M3 - Journal article

C2 - 29708639

VL - 20

SP - 2483

EP - 2499

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 7

ER -