The cytotoxicity of fatty acid/α-lactalbumin complexes depends on the amount and type of fatty acid

Christel Rothe Brinkmann, André Brodkorb, Steffen Thiel, Joseph J. Kehoe*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

15 Citations (Scopus)

Abstract

Complexes of the milk protein, α-lactalbumin, and the fatty acid, oleic acid, have previously been shown to be cytotoxic. Complexes of α-lactalbumin and five different fatty acids (vaccenic, linoleic, palmitoleic, stearic, and elaidic acid) were prepared and compared to those formed with oleic acid. All complexes were cytotoxic to human promyelocytic leukemia-derived (HL-60) cells but to different degrees depending on the fatty acid. The amount of fatty acid per α-lactalbumin molecule was found to correlate with the cytotoxicity; the higher the number of fatty acids per protein, the more cytotoxic the complex. Importantly, all the tested fatty acids were also found to be cytotoxic on their own in a concentration dependent manner. The cytotoxic effect of complexes between α-lactalbumin and linoleic acid, vaccenic acid, or oleic acid was further investigated using flow cytometry and found to induce cell death resembling apoptosis on Jurkat cells.

Original languageEnglish
JournalEuropean Journal of Lipid Science and Technology
Volume115
Issue6
Pages (from-to)591-600
Number of pages10
ISSN1438-7697
DOIs
Publication statusPublished - 1 Jun 2013

Keywords

  • Alpha lactalbumin
  • Cytotoxicity
  • Fatty acids
  • HAMLET
  • Oleic acid

Fingerprint

Dive into the research topics of 'The cytotoxicity of fatty acid/α-lactalbumin complexes depends on the amount and type of fatty acid'. Together they form a unique fingerprint.

Cite this