TY - JOUR
T1 - The arterial blood sampling associated with PET imaging studies can lead to post-scan complications in Göttingen minipigs
AU - Alstrup, Aage Kristian Olsen
AU - Lillethorup, Thea Pinholt
AU - Landau, Anne
AU - Afzelius, Pia
PY - 2024/6
Y1 - 2024/6
N2 - Göttingen minipigs, due to their low adult body weight, are frequently used for longitudinal imaging studies investigating disease progression or treatment effects. We recently showed that prolonged anesthesia, with intensive blood sampling and road transportation, affects internal organs in non-recovery positron emission tomography (PET) imaging studies in domestic pigs. In the current study, we examined if repeated non-invasive PET scans per se affect internal organs using computed tomography (CT). We then examined post-scan complications using clinical observations following PET imaging of Göttingen minipigs with (n=14) and without (n=4) invasive blood sampling. We report that non-invasive CT scans show no organ damage in minipigs (n=4) placed in sternal recumbence for PET scanning last-ing a few hours. Furthermore, upon reviewing medical records of other Göttingen minipigs positioned in sternal recumbence during PET scans, two cases (5 % of scans) with minor post-scan complications were found during the first two weeks after scanning. Minipigs in dorsal recumbence with the surgical placement of femoral catheters for blood sampling were more frequently (6 cases, 43 % of scans) associated with minor-to-moderate post-scan complications. A reduced daily gain in body weight was also observed after minipigs were placed in dorsal recumbence with blood sampling compared to pre-scan daily weight gain. The results suggest anesthetized Göttingen minipigs are somewhat affected by short-term PET scanning procedures, and blood sampling should be reduced when possible. Post-operative care should be improved due to the higher incidence of post-scan complications in minipigs with femo-ral artery catheterization.
AB - Göttingen minipigs, due to their low adult body weight, are frequently used for longitudinal imaging studies investigating disease progression or treatment effects. We recently showed that prolonged anesthesia, with intensive blood sampling and road transportation, affects internal organs in non-recovery positron emission tomography (PET) imaging studies in domestic pigs. In the current study, we examined if repeated non-invasive PET scans per se affect internal organs using computed tomography (CT). We then examined post-scan complications using clinical observations following PET imaging of Göttingen minipigs with (n=14) and without (n=4) invasive blood sampling. We report that non-invasive CT scans show no organ damage in minipigs (n=4) placed in sternal recumbence for PET scanning last-ing a few hours. Furthermore, upon reviewing medical records of other Göttingen minipigs positioned in sternal recumbence during PET scans, two cases (5 % of scans) with minor post-scan complications were found during the first two weeks after scanning. Minipigs in dorsal recumbence with the surgical placement of femoral catheters for blood sampling were more frequently (6 cases, 43 % of scans) associated with minor-to-moderate post-scan complications. A reduced daily gain in body weight was also observed after minipigs were placed in dorsal recumbence with blood sampling compared to pre-scan daily weight gain. The results suggest anesthetized Göttingen minipigs are somewhat affected by short-term PET scanning procedures, and blood sampling should be reduced when possible. Post-operative care should be improved due to the higher incidence of post-scan complications in minipigs with femo-ral artery catheterization.
KW - PET
KW - animals
KW - scanning
UR - http://www.scopus.com/inward/record.url?scp=85196407056&partnerID=8YFLogxK
U2 - 10.23675/sjlas.v50i.22397
DO - 10.23675/sjlas.v50i.22397
M3 - Journal article
SN - 0901-3393
VL - 50
SP - 6
EP - 12
JO - Scandinavian Journal of Laboratory Animal Science
JF - Scandinavian Journal of Laboratory Animal Science
IS - 2
ER -