Abstract
A plant gene transfer system was developed from the Agrobacterium rhizogenes pRi15834 TL-DNA region. "Intermediate integration vectors" constructed from ColE1-derived plasmids served as cloning vectors in Escherichia coli and formed cointegrates into the TL-DNA after transfer to A. rhizogenes. An A. rhizogenes strain with pBR322 plasmid sequences replacing part of the TL-DNA was also constructed. Plasmids unable to replicate in Agrobacterium can integrate into this TL-DNA by homologous recombination through pBR322 sequences. No loss of pathogenicity was observed with the strains formed after integration of intermediate vectors or strains carrying pBR322 in the TL-DNA segment. Up to 15 kb of DNA have been transferred to plant cells with these systems. The T-DNA from a binary vector was cotransformed into hairy roots which developed after transfer of the wild-type pRi T-DNA. Tested on Lotus corniculatus the TL-derived vector system transformed 90% of the developed roots and the T-DNA from the binary vector was cotransformed into 60% of the roots. Minimum copy numbers of one to five were found. Both constitutive and organ-specific plant genes were faithfully expressed after transfer to the legume L. corniculatus.
| Original language | English |
|---|---|
| Journal | MGG Molecular & General Genetics |
| Volume | 207 |
| Issue | 2-3 |
| Pages (from-to) | 251-255 |
| Number of pages | 5 |
| ISSN | 0026-3925 |
| DOIs | |
| Publication status | Published - May 1987 |
| Externally published | Yes |
Keywords
- Agrobacterium rhizogenes
- Binary vectors
- Intermediate integration vectors
- Plant transformation