The α4 isoform of the Na+ ,K+ -ATPase is tuned for changing extracellular environments

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The α4 isoform of the Na+ ,K+ -ATPase is tuned for changing extracellular environments. / Voldsgaard Clausen, Michael; Nissen, Poul; Poulsen, Hanne.

In: The F E B S Journal (Online), Vol. 283, No. 2, 2016, p. 282-293.

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@article{6d5814ef55e940f9a7c25453b69a855d,
title = "The α4 isoform of the Na+ ,K+ -ATPase is tuned for changing extracellular environments",
abstract = "In their journey from the male to the female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope with the associated challenges, spermatozoa express a distinct set of transporters, channels and pumps. One of the membrane proteins unique to spermatozoa is the α4 isoform of the Na(+) ,K(+) -ATPase. In addition to α4, spermatozoa express the ubiquous α1 variant. To get a detailed understanding of how α1 and α4 differ, and why spermatozoa need an additional Na(+) ,K(+) -ATPase, we have conducted an electrophysiological comparison of the rodent isoforms (rat α4 versus mouse α1-3) using the Xenopus oocyte expression system. We demonstrate isoform-specific differences in the voltage sensitivity of steady-state turnover, with α2 being the more sensitive, and α1 and α2 having faster Na(+) release kinetics than α3 and α4. Our data further show that the α1 and α2 turnover rates are fast compared with those of α3 and α4. Finally, α4 is less influenced by changes in extracellular Na(+) and temperature than α1. Based on these findings, we discuss the possibility that evolution has selected robust activity rather than rapid turnover for α4.",
author = "{Voldsgaard Clausen}, Michael and Poul Nissen and Hanne Poulsen",
note = "{\textcopyright} 2015 FEBS.",
year = "2016",
doi = "10.1111/febs.13567",
language = "English",
volume = "283",
pages = "282--293",
journal = "The F E B S Journal (Online)",
issn = "1742-4658",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - The α4 isoform of the Na+ ,K+ -ATPase is tuned for changing extracellular environments

AU - Voldsgaard Clausen, Michael

AU - Nissen, Poul

AU - Poulsen, Hanne

N1 - © 2015 FEBS.

PY - 2016

Y1 - 2016

N2 - In their journey from the male to the female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope with the associated challenges, spermatozoa express a distinct set of transporters, channels and pumps. One of the membrane proteins unique to spermatozoa is the α4 isoform of the Na(+) ,K(+) -ATPase. In addition to α4, spermatozoa express the ubiquous α1 variant. To get a detailed understanding of how α1 and α4 differ, and why spermatozoa need an additional Na(+) ,K(+) -ATPase, we have conducted an electrophysiological comparison of the rodent isoforms (rat α4 versus mouse α1-3) using the Xenopus oocyte expression system. We demonstrate isoform-specific differences in the voltage sensitivity of steady-state turnover, with α2 being the more sensitive, and α1 and α2 having faster Na(+) release kinetics than α3 and α4. Our data further show that the α1 and α2 turnover rates are fast compared with those of α3 and α4. Finally, α4 is less influenced by changes in extracellular Na(+) and temperature than α1. Based on these findings, we discuss the possibility that evolution has selected robust activity rather than rapid turnover for α4.

AB - In their journey from the male to the female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope with the associated challenges, spermatozoa express a distinct set of transporters, channels and pumps. One of the membrane proteins unique to spermatozoa is the α4 isoform of the Na(+) ,K(+) -ATPase. In addition to α4, spermatozoa express the ubiquous α1 variant. To get a detailed understanding of how α1 and α4 differ, and why spermatozoa need an additional Na(+) ,K(+) -ATPase, we have conducted an electrophysiological comparison of the rodent isoforms (rat α4 versus mouse α1-3) using the Xenopus oocyte expression system. We demonstrate isoform-specific differences in the voltage sensitivity of steady-state turnover, with α2 being the more sensitive, and α1 and α2 having faster Na(+) release kinetics than α3 and α4. Our data further show that the α1 and α2 turnover rates are fast compared with those of α3 and α4. Finally, α4 is less influenced by changes in extracellular Na(+) and temperature than α1. Based on these findings, we discuss the possibility that evolution has selected robust activity rather than rapid turnover for α4.

U2 - 10.1111/febs.13567

DO - 10.1111/febs.13567

M3 - Journal article

C2 - 26476261

VL - 283

SP - 282

EP - 293

JO - The F E B S Journal (Online)

JF - The F E B S Journal (Online)

SN - 1742-4658

IS - 2

ER -