The α2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish

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The α2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish. / Doganli, Canan; Kjaer-Sørensen, Kasper; Knoeckel, Christopher; Beck, Hans Christian; Nyengaard, Jens Randel; Honoré, Bent; Nissen, Poul; Ribera, Angela; Oxvig, Claus; Lykke-Hartmann, Karin.

In: Journal of Cell Science, Vol. 125, 12.2012, p. 6166-6175.

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Doganli, Canan ; Kjaer-Sørensen, Kasper ; Knoeckel, Christopher ; Beck, Hans Christian ; Nyengaard, Jens Randel ; Honoré, Bent ; Nissen, Poul ; Ribera, Angela ; Oxvig, Claus ; Lykke-Hartmann, Karin. / The α2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish. In: Journal of Cell Science. 2012 ; Vol. 125. pp. 6166-6175.

Bibtex

@article{37e2ada46f3d42fd8752b0cbd66bf111,
title = "The α2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish",
abstract = "The Na+/K+-ATPase generates ion gradients across the plasma membrane, essential for multiple cellular functions. In mammals, four different Na+/K+-ATPase α-subunit isoforms are associated with characteristic cell-type expression profiles and kinetics. We found the zebrafish α2Na+/K+-ATPase associated with striated muscles and that α2Na+/K+-ATPase knockdown causes a significant depolarization of the resting membrane potential in slow-twitch fibers of skeletal muscles. Abrupt mechanosensory responses were observed in α2Na+/K+-ATPase deficient embryos, possibly linked to a postsynaptic defect. The α2Na+/K+-ATPase deficiency reduced the heart rate and caused a loss of left-right asymmetry in the heart tube. Similar phenotypes observed by knockdown of the Na+/Ca2+ exchanger indicated a role for the interplay between these two proteins on the observed phenotypes. Furthermore, proteomics identified up- and down-regulation of specific phenotype-related proteins, such as parvalbumin, CaM, GFAP and multiple kinases, thus highlighting a potential proteome change associated with the dynamics of α2Na+/K+-ATPase. Taken together, our findings display that zebrafish α2Na+/K+-ATPase is important for skeletal and heart muscle functions",
author = "Canan Doganli and Kasper Kjaer-S{\o}rensen and Christopher Knoeckel and Beck, {Hans Christian} and Nyengaard, {Jens Randel} and Bent Honor{\'e} and Poul Nissen and Angela Ribera and Claus Oxvig and Karin Lykke-Hartmann",
year = "2012",
month = dec,
doi = "10.1242/jcs.115808",
language = "English",
volume = "125",
pages = "6166--6175",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",

}

RIS

TY - JOUR

T1 - The α2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish

AU - Doganli, Canan

AU - Kjaer-Sørensen, Kasper

AU - Knoeckel, Christopher

AU - Beck, Hans Christian

AU - Nyengaard, Jens Randel

AU - Honoré, Bent

AU - Nissen, Poul

AU - Ribera, Angela

AU - Oxvig, Claus

AU - Lykke-Hartmann, Karin

PY - 2012/12

Y1 - 2012/12

N2 - The Na+/K+-ATPase generates ion gradients across the plasma membrane, essential for multiple cellular functions. In mammals, four different Na+/K+-ATPase α-subunit isoforms are associated with characteristic cell-type expression profiles and kinetics. We found the zebrafish α2Na+/K+-ATPase associated with striated muscles and that α2Na+/K+-ATPase knockdown causes a significant depolarization of the resting membrane potential in slow-twitch fibers of skeletal muscles. Abrupt mechanosensory responses were observed in α2Na+/K+-ATPase deficient embryos, possibly linked to a postsynaptic defect. The α2Na+/K+-ATPase deficiency reduced the heart rate and caused a loss of left-right asymmetry in the heart tube. Similar phenotypes observed by knockdown of the Na+/Ca2+ exchanger indicated a role for the interplay between these two proteins on the observed phenotypes. Furthermore, proteomics identified up- and down-regulation of specific phenotype-related proteins, such as parvalbumin, CaM, GFAP and multiple kinases, thus highlighting a potential proteome change associated with the dynamics of α2Na+/K+-ATPase. Taken together, our findings display that zebrafish α2Na+/K+-ATPase is important for skeletal and heart muscle functions

AB - The Na+/K+-ATPase generates ion gradients across the plasma membrane, essential for multiple cellular functions. In mammals, four different Na+/K+-ATPase α-subunit isoforms are associated with characteristic cell-type expression profiles and kinetics. We found the zebrafish α2Na+/K+-ATPase associated with striated muscles and that α2Na+/K+-ATPase knockdown causes a significant depolarization of the resting membrane potential in slow-twitch fibers of skeletal muscles. Abrupt mechanosensory responses were observed in α2Na+/K+-ATPase deficient embryos, possibly linked to a postsynaptic defect. The α2Na+/K+-ATPase deficiency reduced the heart rate and caused a loss of left-right asymmetry in the heart tube. Similar phenotypes observed by knockdown of the Na+/Ca2+ exchanger indicated a role for the interplay between these two proteins on the observed phenotypes. Furthermore, proteomics identified up- and down-regulation of specific phenotype-related proteins, such as parvalbumin, CaM, GFAP and multiple kinases, thus highlighting a potential proteome change associated with the dynamics of α2Na+/K+-ATPase. Taken together, our findings display that zebrafish α2Na+/K+-ATPase is important for skeletal and heart muscle functions

U2 - 10.1242/jcs.115808

DO - 10.1242/jcs.115808

M3 - Journal article

C2 - 23097043

VL - 125

SP - 6166

EP - 6175

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -