The α4 isoform of the Na⁺ ,K⁺ -ATPase is tuned for changing extracellular environments

TY - JOUR

T1 - The α4 isoform of the Na+ ,K+ -ATPase is tuned for changing extracellular environments

AU - Voldsgaard Clausen, Michael

AU - Nissen, Poul

AU - Poulsen, Hanne

N1 - © 2015 FEBS.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - In their journey from the male to the female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope with the associated challenges, spermatozoa express a distinct set of transporters, channels and pumps. One of the membrane proteins unique to spermatozoa is the α4 isoform of the Na +,K +-ATPase. In addition to α4, spermatozoa express the ubiquous α1 variant. To get a detailed understanding of how α1 and α4 differ, and why spermatozoa need an additional Na +,K +-ATPase, we have conducted an electrophysiological comparison of the rodent isoforms (rat α4 versus mouse α1-3) using the Xenopus oocyte expression system. We demonstrate isoform-specific differences in the voltage sensitivity of steady-state turnover, with α2 being the more sensitive, and α1 and α2 having faster Na + release kinetics than α3 and α4. Our data further show that the α1 and α2 turnover rates are fast compared with those of α3 and α4. Finally, α4 is less influenced by changes in extracellular Na + and temperature than α1. Based on these findings, we discuss the possibility that evolution has selected robust activity rather than rapid turnover for α4. Sperm cells express the α1 and α4 isoforms of the Na +,K +-ATPase. Here we demonstrate that compared with α1, α4 has a slow turnover rate, but is less affected by changes in voltage, external sodium and temperature. We suggest that with α4, evolution may have selected stability over turnover rate.

AB - In their journey from the male to the female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope with the associated challenges, spermatozoa express a distinct set of transporters, channels and pumps. One of the membrane proteins unique to spermatozoa is the α4 isoform of the Na +,K +-ATPase. In addition to α4, spermatozoa express the ubiquous α1 variant. To get a detailed understanding of how α1 and α4 differ, and why spermatozoa need an additional Na +,K +-ATPase, we have conducted an electrophysiological comparison of the rodent isoforms (rat α4 versus mouse α1-3) using the Xenopus oocyte expression system. We demonstrate isoform-specific differences in the voltage sensitivity of steady-state turnover, with α2 being the more sensitive, and α1 and α2 having faster Na + release kinetics than α3 and α4. Our data further show that the α1 and α2 turnover rates are fast compared with those of α3 and α4. Finally, α4 is less influenced by changes in extracellular Na + and temperature than α1. Based on these findings, we discuss the possibility that evolution has selected robust activity rather than rapid turnover for α4. Sperm cells express the α1 and α4 isoforms of the Na +,K +-ATPase. Here we demonstrate that compared with α1, α4 has a slow turnover rate, but is less affected by changes in voltage, external sodium and temperature. We suggest that with α4, evolution may have selected stability over turnover rate.

KW - Na ,K -ATPase

KW - adaptation

KW - isoforms

KW - sperm

KW - voltage dependence

UR - http://www.scopus.com/inward/record.url?scp=84957445199&partnerID=8YFLogxK

U2 - 10.1111/febs.13567

DO - 10.1111/febs.13567

M3 - Journal article

C2 - 26476261

SN - 1742-4658

VL - 283

SP - 282

EP - 293

JO - FEBS journal

JF - FEBS journal

IS - 2

ER -