Temporal changes in glycogenolytic enzyme mRNAs during myogenesis of primary porcine satellite cells

TY - JOUR

T1 - Temporal changes in glycogenolytic enzyme mRNAs during myogenesis of primary porcine satellite cells

AU - Henckel, Poul

AU - Theil, Peter Kappel

AU - Sørensen, Inge Lise

AU - Oksbjerg, Niels

PY - 2007

Y1 - 2007

N2 - The objective was to study the regulation of glycogenolytic enzyme mRNAs in porcine satellite cells during proliferation and differentiation. Beyond 80% confluence, cells were grown in absence or presence of 1 lM insulin. The observed increases in abundance of mRNA for glycogenin, glycogen synthase, phosphorylase kinase, phosphorylase and glycogen debranching enzyme, and no alterations of the transporter molecule GLUT4, clearly indicate that glycogenolytic enzymes of potential importance to meat quality development are regulated at the gene level during myogenesis, and are heavily involved in muscle cell and muscle fibre development. The genes, however, are not influenced by insulin, and the lack of response to insulin of expression of gene-encoding enzymes involved in the formation and degradation of glycogen may question the applicability of porcine cell culture systems, like the one applied, as a model to study the regulation and regulatory mechanism of energy metabolism in muscles.

AB - The objective was to study the regulation of glycogenolytic enzyme mRNAs in porcine satellite cells during proliferation and differentiation. Beyond 80% confluence, cells were grown in absence or presence of 1 lM insulin. The observed increases in abundance of mRNA for glycogenin, glycogen synthase, phosphorylase kinase, phosphorylase and glycogen debranching enzyme, and no alterations of the transporter molecule GLUT4, clearly indicate that glycogenolytic enzymes of potential importance to meat quality development are regulated at the gene level during myogenesis, and are heavily involved in muscle cell and muscle fibre development. The genes, however, are not influenced by insulin, and the lack of response to insulin of expression of gene-encoding enzymes involved in the formation and degradation of glycogen may question the applicability of porcine cell culture systems, like the one applied, as a model to study the regulation and regulatory mechanism of energy metabolism in muscles.

KW - gene expression

KW - Pig

KW - Satellite cells

KW - Glycogenolytic enzymes

KW - Gene expression

M3 - Journal article

SN - 0309-1740

VL - 75

SP - 248

EP - 255

JO - Meat Science

JF - Meat Science

ER -