Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching

Michael W. Haydell, Mathias Centola, Volker Adam, Julian Valero Moreno*, Michael Famulok*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.
Original languageEnglish
JournalJournal of the American Chemical Society
Pages (from-to)16868-16872
Number of pages5
Publication statusPublished - Nov 2018
Externally publishedYes


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