Temperature-induced ultradense PEG polyelectrolyte surface grafting provides effective long-term bioresistance against mammalian cells, serum, and whole blood

TY - JOUR

T1 - Temperature-induced ultradense PEG polyelectrolyte surface grafting provides effective long-term bioresistance against mammalian cells, serum, and whole blood

AU - Ogaki, Ryosuke

AU - Andersen, Ole Zoffmann

AU - Jensen, G.V.

AU - Kolind, Kristian

AU - Kraft, D.C.E.

AU - Pedersen, Jan Skov

AU - Foss, M.

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/11/13

Y1 - 2012/11/13

N2 - We report a facile method of generating ultradense poly(l-lysine)-graft- poly(ethylene glycol) (PLL-g-PEG) surface by using high temperature alone, which in turn provides dramatic improvement in resisting nonspecific bioadsorption. X-ray photoelectron spectroscopy (XPS) revealed that the surface graft density increased ∼4 times higher on the surface prepared at 80 °C compared to 20 °C. The studies from small-angle X-ray scattering (SAXS) and the effect of varying ionic strength during/post assemblies at 20 and 80 °C indicated that the "cloud point grafting effect" is not the cause for obtaining high density grafting. Stringent long-term bioresistance tests have been conducted and the temperature-induced PLL-g-PEG surfaces have achieved (1) zero mammalian cell adsorption/migration for up to 36 days and (2) extremely close-to-zero protein adsorptions have been observed even after 36 days in 10% serum media and 24 h in whole blood within the ultrasensitive detection limit of time-of-flight secondary ion mass spectrometry (ToF-SIMS).

AB - We report a facile method of generating ultradense poly(l-lysine)-graft- poly(ethylene glycol) (PLL-g-PEG) surface by using high temperature alone, which in turn provides dramatic improvement in resisting nonspecific bioadsorption. X-ray photoelectron spectroscopy (XPS) revealed that the surface graft density increased ∼4 times higher on the surface prepared at 80 °C compared to 20 °C. The studies from small-angle X-ray scattering (SAXS) and the effect of varying ionic strength during/post assemblies at 20 and 80 °C indicated that the "cloud point grafting effect" is not the cause for obtaining high density grafting. Stringent long-term bioresistance tests have been conducted and the temperature-induced PLL-g-PEG surfaces have achieved (1) zero mammalian cell adsorption/migration for up to 36 days and (2) extremely close-to-zero protein adsorptions have been observed even after 36 days in 10% serum media and 24 h in whole blood within the ultrasensitive detection limit of time-of-flight secondary ion mass spectrometry (ToF-SIMS).

UR - http://www.scopus.com/inward/record.url?scp=84869070303&partnerID=8YFLogxK

U2 - 10.1021/bm301125g

DO - 10.1021/bm301125g

M3 - Journal article

C2 - 23082770

AN - SCOPUS:84869070303

SN - 1525-7797

VL - 13

SP - 3668

EP - 3677

JO - Biomacromolecules

JF - Biomacromolecules

IS - 11

ER -