Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

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Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. / Hansen, Line; Unmack Larsen, Esben Kjær; Nielsen, Erik Holm; Iversen, Frank; Liu, Zhuo; Thomsen, Karen; Pedersen, Michael; Skrydstrup, Troels; Nielsen, Niels Christian; Ploug, Michael; Kjems, Jørgen.

In: Nanoscale, Vol. 5, 09.07.2013, p. 8192-8201.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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APA

Hansen, L., Unmack Larsen, E. K., Nielsen, E. H., Iversen, F., Liu, Z., Thomsen, K., ... Kjems, J. (2013). Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. Nanoscale, 5, 8192-8201. https://doi.org/10.1039/c3nr32922d

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MLA

Vancouver

Hansen L, Unmack Larsen EK, Nielsen EH, Iversen F, Liu Z, Thomsen K et al. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. Nanoscale. 2013 Jul 9;5:8192-8201. https://doi.org/10.1039/c3nr32922d

Author

Hansen, Line ; Unmack Larsen, Esben Kjær ; Nielsen, Erik Holm ; Iversen, Frank ; Liu, Zhuo ; Thomsen, Karen ; Pedersen, Michael ; Skrydstrup, Troels ; Nielsen, Niels Christian ; Ploug, Michael ; Kjems, Jørgen. / Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. In: Nanoscale. 2013 ; Vol. 5. pp. 8192-8201.

Bibtex

@article{7804499a617a4f89adf7ac9de52b0060,
title = "Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells",
abstract = "Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.",
author = "Line Hansen and {Unmack Larsen}, {Esben Kj{\ae}r} and Nielsen, {Erik Holm} and Frank Iversen and Zhuo Liu and Karen Thomsen and Michael Pedersen and Troels Skrydstrup and Nielsen, {Niels Christian} and Michael Ploug and J{\o}rgen Kjems",
year = "2013",
month = "7",
day = "9",
doi = "10.1039/c3nr32922d",
language = "English",
volume = "5",
pages = "8192--8201",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "ROYAL SOC CHEMISTRY",

}

RIS

TY - JOUR

T1 - Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

AU - Hansen, Line

AU - Unmack Larsen, Esben Kjær

AU - Nielsen, Erik Holm

AU - Iversen, Frank

AU - Liu, Zhuo

AU - Thomsen, Karen

AU - Pedersen, Michael

AU - Skrydstrup, Troels

AU - Nielsen, Niels Christian

AU - Ploug, Michael

AU - Kjems, Jørgen

PY - 2013/7/9

Y1 - 2013/7/9

N2 - Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.

AB - Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.

U2 - 10.1039/c3nr32922d

DO - 10.1039/c3nr32922d

M3 - Journal article

C2 - 23835641

VL - 5

SP - 8192

EP - 8201

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

ER -