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Targeted proteomics as a tool for porcine acute phase proteins measurements

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Targeted proteomics as a tool for porcine acute phase proteins measurements. / Marco-Ramell, Anna; Bassols, Anna; Bislev, Stine Lønnerup; Sanggaard, Kristian Wejse; Enghild, Jan Johannes; Bendixen, Emøke; Fraile, Lorenzo.

Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. ed. / André de Almeida ; David Eckersall ; Elena Bencurova ; Saskia Dolinska; Patrik Mlynarcik ; Miroslava Vincova ; Mangesh Bhide . Wageningen Academic Publishers, 2013. p. 217-220.

Research output: Contribution to book/anthology/report/proceedingArticle in proceedingsResearchpeer-review

Harvard

Marco-Ramell, A, Bassols, A, Bislev, SL, Sanggaard, KW, Enghild, JJ, Bendixen, E & Fraile, L 2013, Targeted proteomics as a tool for porcine acute phase proteins measurements. in AD Almeida , D Eckersall , E Bencurova , S Dolinska, P Mlynarcik , M Vincova & M Bhide (eds), Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. Wageningen Academic Publishers, pp. 217-220, Farm animal proteomics 2013, Košice, Slovakia, 25/04/2013. https://doi.org/10.3920/978-90-8686-776-9_55

APA

Marco-Ramell, A., Bassols, A., Bislev, S. L., Sanggaard, K. W., Enghild, J. J., Bendixen, E., & Fraile, L. (2013). Targeted proteomics as a tool for porcine acute phase proteins measurements. In A. D. Almeida , D. Eckersall , E. Bencurova , S. Dolinska, P. Mlynarcik , M. Vincova , & M. Bhide (Eds.), Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002 (pp. 217-220). Wageningen Academic Publishers. https://doi.org/10.3920/978-90-8686-776-9_55

CBE

Marco-Ramell A, Bassols A, Bislev SL, Sanggaard KW, Enghild JJ, Bendixen E, Fraile L. 2013. Targeted proteomics as a tool for porcine acute phase proteins measurements. Almeida AD, Eckersall D, Bencurova E, Dolinska S, Mlynarcik P, Vincova M, Bhide M, editors. In Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. Wageningen Academic Publishers. pp. 217-220. https://doi.org/10.3920/978-90-8686-776-9_55

MLA

Marco-Ramell, Anna et al. "Targeted proteomics as a tool for porcine acute phase proteins measurements"., Almeida , André de, Eckersall , David, Bencurova , Elena Dolinska, Saskia Mlynarcik , Patrik Vincova , Miroslava Bhide , Mangesh (editors). Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. Chapter IV, Wageningen Academic Publishers. 2013, 217-220. https://doi.org/10.3920/978-90-8686-776-9_55

Vancouver

Marco-Ramell A, Bassols A, Bislev SL, Sanggaard KW, Enghild JJ, Bendixen E et al. Targeted proteomics as a tool for porcine acute phase proteins measurements. In Almeida AD, Eckersall D, Bencurova E, Dolinska S, Mlynarcik P, Vincova M, Bhide M, editors, Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. Wageningen Academic Publishers. 2013. p. 217-220 https://doi.org/10.3920/978-90-8686-776-9_55

Author

Marco-Ramell, Anna ; Bassols, Anna ; Bislev, Stine Lønnerup ; Sanggaard, Kristian Wejse ; Enghild, Jan Johannes ; Bendixen, Emøke ; Fraile, Lorenzo. / Targeted proteomics as a tool for porcine acute phase proteins measurements. Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002. editor / André de Almeida ; David Eckersall ; Elena Bencurova ; Saskia Dolinska ; Patrik Mlynarcik ; Miroslava Vincova ; Mangesh Bhide . Wageningen Academic Publishers, 2013. pp. 217-220

Bibtex

@inproceedings{9d8c884423d24cbe93a47b58221f0cad,
title = "Targeted proteomics as a tool for porcine acute phase proteins measurements",
abstract = "It is mandatory, after protein identification, to validate candidate markers in a larger amount of samples for biomarker discovery. The most used methods for validation of new markers are enzymatic or immunoassay-based commercial kits. However, relatively few kits are available for farm animals. Selected reaction monitoring (SRM), a targeted quantitative proteomic technique, may be used as an alternative to commercial kits for the measurement and validation of target proteins. Acute phase proteins (APPs) are widely recognized inflammation and infection biomarkers (Eckersall, 2010) and there is recent evidence that they can be also considered as welfare markers (Giannetto et al., 2011). During an acute stress situation, the levels of some interleukins (IL-1β, IL-6 and TNF-α) are increased (Elenkov and Chrousos, 2002) and consequently the concentration of the APPs varies dramatically. In pigs the most important APPs include haptoglobin (Hp), C-reactive protein (CRP), the inter-α-inhibitor-heavy chain 4 (ITIH4, also called Pig major acute phase protein, or Pig-MAP), serum amyloid A (SAA) and apolipoprotein A-I (Apo A-I), but also other proteins are well known to mark the acute phase response, as albumin, transferrin (Heegaard et al., 2011) and fetuin A (Brown et al., 1992).",
author = "Anna Marco-Ramell and Anna Bassols and Bislev, {Stine L{\o}nnerup} and Sanggaard, {Kristian Wejse} and Enghild, {Jan Johannes} and Em{\o}ke Bendixen and Lorenzo Fraile",
year = "2013",
month = apr,
day = "25",
doi = "10.3920/978-90-8686-776-9_55",
language = "English",
pages = "217--220",
editor = "{Almeida }, {Andr{\'e} de} and {Eckersall }, David and {Bencurova }, {Elena } and Saskia Dolinska and {Mlynarcik }, Patrik and {Vincova }, Miroslava and {Bhide }, Mangesh",
booktitle = "Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002",
publisher = "Wageningen Academic Publishers",
note = "Farm animal proteomics 2013 ; Conference date: 25-04-2013 Through 26-04-2013",

}

RIS

TY - GEN

T1 - Targeted proteomics as a tool for porcine acute phase proteins measurements

AU - Marco-Ramell, Anna

AU - Bassols, Anna

AU - Bislev, Stine Lønnerup

AU - Sanggaard, Kristian Wejse

AU - Enghild, Jan Johannes

AU - Bendixen, Emøke

AU - Fraile, Lorenzo

N1 - Conference code: 4th

PY - 2013/4/25

Y1 - 2013/4/25

N2 - It is mandatory, after protein identification, to validate candidate markers in a larger amount of samples for biomarker discovery. The most used methods for validation of new markers are enzymatic or immunoassay-based commercial kits. However, relatively few kits are available for farm animals. Selected reaction monitoring (SRM), a targeted quantitative proteomic technique, may be used as an alternative to commercial kits for the measurement and validation of target proteins. Acute phase proteins (APPs) are widely recognized inflammation and infection biomarkers (Eckersall, 2010) and there is recent evidence that they can be also considered as welfare markers (Giannetto et al., 2011). During an acute stress situation, the levels of some interleukins (IL-1β, IL-6 and TNF-α) are increased (Elenkov and Chrousos, 2002) and consequently the concentration of the APPs varies dramatically. In pigs the most important APPs include haptoglobin (Hp), C-reactive protein (CRP), the inter-α-inhibitor-heavy chain 4 (ITIH4, also called Pig major acute phase protein, or Pig-MAP), serum amyloid A (SAA) and apolipoprotein A-I (Apo A-I), but also other proteins are well known to mark the acute phase response, as albumin, transferrin (Heegaard et al., 2011) and fetuin A (Brown et al., 1992).

AB - It is mandatory, after protein identification, to validate candidate markers in a larger amount of samples for biomarker discovery. The most used methods for validation of new markers are enzymatic or immunoassay-based commercial kits. However, relatively few kits are available for farm animals. Selected reaction monitoring (SRM), a targeted quantitative proteomic technique, may be used as an alternative to commercial kits for the measurement and validation of target proteins. Acute phase proteins (APPs) are widely recognized inflammation and infection biomarkers (Eckersall, 2010) and there is recent evidence that they can be also considered as welfare markers (Giannetto et al., 2011). During an acute stress situation, the levels of some interleukins (IL-1β, IL-6 and TNF-α) are increased (Elenkov and Chrousos, 2002) and consequently the concentration of the APPs varies dramatically. In pigs the most important APPs include haptoglobin (Hp), C-reactive protein (CRP), the inter-α-inhibitor-heavy chain 4 (ITIH4, also called Pig major acute phase protein, or Pig-MAP), serum amyloid A (SAA) and apolipoprotein A-I (Apo A-I), but also other proteins are well known to mark the acute phase response, as albumin, transferrin (Heegaard et al., 2011) and fetuin A (Brown et al., 1992).

U2 - 10.3920/978-90-8686-776-9_55

DO - 10.3920/978-90-8686-776-9_55

M3 - Article in proceedings

SP - 217

EP - 220

BT - Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002

A2 - Almeida , André de

A2 - Eckersall , David

A2 - Bencurova , Elena

A2 - Dolinska, Saskia

A2 - Mlynarcik , Patrik

A2 - Vincova , Miroslava

A2 - Bhide , Mangesh

PB - Wageningen Academic Publishers

T2 - Farm animal proteomics 2013

Y2 - 25 April 2013 through 26 April 2013

ER -