Abstract
T-bodies are localized S. cerevisiae RNPs containing Ty1 retroviral components and speculated to play a role in the assembly of virus-like particles (VLPs). Mapping requirements for T-body formation, we demonstrate that ectopic expression of immature TyA1/Gag (Gag-p49), a structural component of the Ty1 capsid, is sufficient for T-body formation both under normal conditions as well as in a strain background devoid of endogenous Gag. Moreover, T-bodies are readily formed when Ty1 transposition is blocked. Thus, T-bodies represent an early stage in the Ty1 life cycle, preceding VLP maturation
| Original language | English |
|---|---|
| Journal | RNA Biology |
| Volume | 8 |
| Issue | 2 |
| Pages (from-to) | 184-189 |
| Number of pages | 6 |
| ISSN | 1547-6286 |
| DOIs | |
| Publication status | Published - 2011 |
Keywords
- Gag
- T-body
- Transposition
- Ty1
- VLP