Systemic control of legume susceptibility to rhizobial infection by a mobile microRNA

Daniela Tsikou; Zhe Yan; Dennis B. Holt; Nikolaj B. Abel; Dugald E. Reid; Lene H. Madsen; Hemal Bhasin; Moritz Sexauer; Jens Stougaard; Katharina Markmann

doi:10.1126/science.aat6907

Systemic control of legume susceptibility to rhizobial infection by a mobile microRNA

Daniela Tsikou, Zhe Yan, Dennis B. Holt, Nikolaj B. Abel, Dugald E. Reid, Lene H. Madsen, Hemal Bhasin, Moritz Sexauer, Jens Stougaard, Katharina Markmann^*

^*Corresponding author for this work

Department of Molecular Biology and Genetics - Plant Molecular Biology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Nitrogen-fixing root nodules on legumes result from two developmental processes, bacterial infection and nodule organogenesis. To balance symbiosis and plant growth, legume hosts restrict nodule numbers through an inducible autoregulatory process. Here, we present a mechanism where repression of a negative regulator ensures symbiotic susceptibility of uninfected roots of the host Lotus japonicus.We show that microRNA miR2111 undergoes shoot-to-root translocation to control rhizobial infection through posttranscriptional regulation of the symbiosis suppressor TOO MUCH LOVE in roots. MiR2111 maintains a susceptible default status in uninfected hosts and functions as an activator of symbiosis downstream of LOTUS HISTIDINE KINASE1-mediated cytokinin perception in roots and HYPERNODULATION ABERRANT ROOT FORMATION1, a shoot factor in autoregulation. The miR2111-TML node ensures activation of feedback regulation to balance infection and nodulation events.

Original language	English
Journal	Science
Volume	362
Issue	6411
Pages (from-to)	233-236
Number of pages	4
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.aat6907
Publication status	Published - 12 Oct 2018

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Systematic control of legumeAccepted manuscript, 116 MB

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title = "Systemic control of legume susceptibility to rhizobial infection by a mobile microRNA",

abstract = "Nitrogen-fixing root nodules on legumes result from two developmental processes, bacterial infection and nodule organogenesis. To balance symbiosis and plant growth, legume hosts restrict nodule numbers through an inducible autoregulatory process. Here, we present a mechanism where repression of a negative regulator ensures symbiotic susceptibility of uninfected roots of the host Lotus japonicus.We show that microRNA miR2111 undergoes shoot-to-root translocation to control rhizobial infection through posttranscriptional regulation of the symbiosis suppressor TOO MUCH LOVE in roots. MiR2111 maintains a susceptible default status in uninfected hosts and functions as an activator of symbiosis downstream of LOTUS HISTIDINE KINASE1-mediated cytokinin perception in roots and HYPERNODULATION ABERRANT ROOT FORMATION1, a shoot factor in autoregulation. The miR2111-TML node ensures activation of feedback regulation to balance infection and nodulation events.",

author = "Daniela Tsikou and Zhe Yan and Holt, {Dennis B.} and Abel, {Nikolaj B.} and Reid, {Dugald E.} and Madsen, {Lene H.} and Hemal Bhasin and Moritz Sexauer and Jens Stougaard and Katharina Markmann",

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doi = "10.1126/science.aat6907",

language = "English",

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AU - Tsikou, Daniela

AU - Yan, Zhe

AU - Holt, Dennis B.

AU - Abel, Nikolaj B.

AU - Reid, Dugald E.

AU - Madsen, Lene H.

AU - Bhasin, Hemal

AU - Sexauer, Moritz

AU - Stougaard, Jens

AU - Markmann, Katharina

PY - 2018/10/12

Y1 - 2018/10/12

N2 - Nitrogen-fixing root nodules on legumes result from two developmental processes, bacterial infection and nodule organogenesis. To balance symbiosis and plant growth, legume hosts restrict nodule numbers through an inducible autoregulatory process. Here, we present a mechanism where repression of a negative regulator ensures symbiotic susceptibility of uninfected roots of the host Lotus japonicus.We show that microRNA miR2111 undergoes shoot-to-root translocation to control rhizobial infection through posttranscriptional regulation of the symbiosis suppressor TOO MUCH LOVE in roots. MiR2111 maintains a susceptible default status in uninfected hosts and functions as an activator of symbiosis downstream of LOTUS HISTIDINE KINASE1-mediated cytokinin perception in roots and HYPERNODULATION ABERRANT ROOT FORMATION1, a shoot factor in autoregulation. The miR2111-TML node ensures activation of feedback regulation to balance infection and nodulation events.

AB - Nitrogen-fixing root nodules on legumes result from two developmental processes, bacterial infection and nodule organogenesis. To balance symbiosis and plant growth, legume hosts restrict nodule numbers through an inducible autoregulatory process. Here, we present a mechanism where repression of a negative regulator ensures symbiotic susceptibility of uninfected roots of the host Lotus japonicus.We show that microRNA miR2111 undergoes shoot-to-root translocation to control rhizobial infection through posttranscriptional regulation of the symbiosis suppressor TOO MUCH LOVE in roots. MiR2111 maintains a susceptible default status in uninfected hosts and functions as an activator of symbiosis downstream of LOTUS HISTIDINE KINASE1-mediated cytokinin perception in roots and HYPERNODULATION ABERRANT ROOT FORMATION1, a shoot factor in autoregulation. The miR2111-TML node ensures activation of feedback regulation to balance infection and nodulation events.

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VL - 362

SP - 233

EP - 236

JO - Science

JF - Science

IS - 6411

ER -

Systemic control of legume susceptibility to rhizobial infection by a mobile microRNA

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