Aarhus University Seal / Aarhus Universitets segl

Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Ya-Ling Chiu, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States.
  • ,
  • Hon Fai Chan, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States., United States
  • Kyle K L Phua, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States., Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore, United States
  • Ying Zhang, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States., United States
  • Sissel Juul, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States.
  • ,
  • Birgitta R Knudsen
  • Kam W Leong, Department of Biomedical Engineering, Duke University , North Carolina 27708, United States., United States
  • Yi-Ping Ho, Denmark

Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

Original languageEnglish
JournalACS Nano
Volume8
Issue4
Pages (from-to)3913-3920
Number of pages8
ISSN1936-0851
DOIs
Publication statusPublished - 25 Mar 2014

See relations at Aarhus University Citationformats

ID: 73740992