Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications

Ya-Ling Chiu; Hon Fai Chan; Kyle K L Phua; Ying Zhang; Sissel Juul; Birgitta R Knudsen; Kam W Leong; Yi-Ping Ho

doi:10.1021/nn500810n

Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications

Ya-Ling Chiu, Hon Fai Chan, Kyle K L Phua, Ying Zhang, Sissel Juul, Birgitta R Knudsen, Kam W Leong, Yi-Ping Ho

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

59 Citations (Scopus)

Abstract

Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

Original language	English
Journal	ACS Nano
Volume	8
Issue	4
Pages (from-to)	3913-3920
Number of pages	8
ISSN	1936-0851
DOIs	https://doi.org/10.1021/nn500810n
Publication status	Published - 25 Mar 2014

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title = "Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications",

abstract = "Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.",

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T1 - Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications

AU - Chiu, Ya-Ling

AU - Chan, Hon Fai

AU - Phua, Kyle K L

AU - Zhang, Ying

AU - Juul, Sissel

AU - Knudsen, Birgitta R

AU - Leong, Kam W

AU - Ho, Yi-Ping

PY - 2014/3/25

Y1 - 2014/3/25

N2 - Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

AB - Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

U2 - 10.1021/nn500810n

DO - 10.1021/nn500810n

M3 - Journal article

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EP - 3920

JO - ACS Nano

JF - ACS Nano

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ER -

Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications

Abstract

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