Abstract
The biological interactions between plants and their root microbiomes are essential for plant growth, and even though plant genotype [G], soil microbiome [M], and growth conditions (environment) [E] are the core factors shaping root microbiome, their relationships remain unclear. In this study we investigated the effects of G, M, and E and their interactions on the Lotus root microbiome and plant growth using an in vitro cross-inoculation approach which reconstructed the interactions between nine Lotus accessions and four soil microbiomes under two different environmental conditions. Results suggested that a large proportion of the root microbiome composition is determined by M and E, while G-related (G, G × M, and G × E) effects were significant but small. In contrast, the interaction between G and M had a more pronounced effect on plant shoot growth than M alone. Our findings also indicated that most microbiome variations controlled by M have little effect on plant phenotypes, whereas G × M interactions have more significant effects. Plant genotype-dependent interactions with soil microbes warrant more attention to optimize crop yield and resilience.
| Original language | English |
|---|---|
| Article number | fiae056 |
| Journal | FEMS Microbiology Ecology |
| Volume | 100 |
| Issue | 5 |
| Number of pages | 12 |
| ISSN | 0168-6496 |
| DOIs | |
| Publication status | Published - May 2024 |
Keywords
- 16S rRNA sequencing
- Lotus japonicus
- cross-inoculation experiment
- growth environment
- plant–microbiome interaction
- root microbiome
- Lotus/microbiology
- Plant Roots/microbiology
- Soil/chemistry
- Soil Microbiology
- Genotype
- Microbiota/genetics