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SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features

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  • Lucas Gewehr, Johannes Gutenberg University Mainz
  • ,
  • Benedikt Junglas, Forschungszentrum Jülich (FZJ)
  • ,
  • Ruven Jilly, Johannes Gutenberg University Mainz
  • ,
  • Johannes Franz, Max Planck Institute for Polymer Research
  • ,
  • Wenyu Eva Zhu, Johannes Gutenberg University Mainz
  • ,
  • Tobias Weidner
  • Mischa Bonn, Max Planck Institute for Polymer Research
  • ,
  • Carsten Sachse, Forschungszentrum Jülich (FZJ), Heinrich Heine University Düsseldorf
  • ,
  • Dirk Schneider, Johannes Gutenberg University Mainz

Dynamin-like proteins are membrane remodeling GTPases with well-understood functions in eukaryotic cells. However, bacterial dynamin-like proteins are still poorly investigated. SynDLP, the dynamin-like protein of the cyanobacterium Synechocystis sp. PCC 6803, forms ordered oligomers in solution. The 3.7 Å resolution cryo-EM structure of SynDLP oligomers reveals the presence of oligomeric stalk interfaces typical for eukaryotic dynamin-like proteins. The bundle signaling element domain shows distinct features, such as an intramolecular disulfide bridge that affects the GTPase activity, or an expanded intermolecular interface with the GTPase domain. In addition to typical GD-GD contacts, such atypical GTPase domain interfaces might be a GTPase activity regulating tool in oligomerized SynDLP. Furthermore, we show that SynDLP interacts with and intercalates into membranes containing negatively charged thylakoid membrane lipids independent of nucleotides. The structural characteristics of SynDLP oligomers suggest it to be the closest known bacterial ancestor of eukaryotic dynamin.

Original languageEnglish
Article number2156
JournalNature Communications
Number of pages17
Publication statusPublished - Apr 2023

Bibliographical note

© 2023. The Author(s).

    Research areas

  • Synechocystis/genetics, Eukaryota/metabolism, Eukaryotic Cells/metabolism, Dynamins/metabolism, GTP Phosphohydrolases/genetics, Thylakoids/metabolism, Bacterial Proteins/genetics

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