Surgical Repair of Annulus Defect with Biomimetic Multilamellar Nano/microfibrous Scaffold in a Porcine Model

Ran Kang, Haisheng Li, Xizhi Peng, Steffen Ringgaard, Anette Baatrup, Kresten Rickers, Ming Sun, Dang Quang Svend Le, Miao Wang, Lin Xie, Menglin Chen, Cody Bünger

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Annulus defect is associated with reherniation and disc degeneration after discectomy; currently there is no effective treatment that addresses this problem. The annulus is a hierarchical lamellar structure, where each lamella consists of aligned collagen fibres, which are parallel and tilted at 30° to the spinal axis. In this study, a biomimetic biodegradable scaffold consisting of multilamellar nano/microfibres, sharing nanotopography and microporosity similar to the native lamellar structure, was assessed in a porcine model, aided by sealing with fascia and medical glue and subsequent suture fixation. After 6- and 12-week observation, we found that this treatment restored nucleus volume and slowed down disc degeneration, as indicated by magnetic resonance imaging of T1/T2-weighted, T2-mapping, T1-ρ imaging. Histological analysis showed aligned collagen fibres organized in the scaffold and integrated with surrounding native annulus tissue. The autologous bone marrow concentrate-seeded scaffolds showed slightly earlier collagen fibre formation at 6 weeks. This novel treatment could efficiently close the annulus defect with newly formed, organized and integrated collagen fibres in a porcine model.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue1
Pages (from-to)164-174
Number of pages11
ISSN1932-6254
DOIs
Publication statusPublished - Jan 2018

Keywords

  • ANULAR REPAIR
  • BACK-PAIN
  • FIBROSUS
  • HEIGHT LOSS
  • HERNIATION
  • INTERVERTEBRAL DISC DEGENERATION
  • LUMBAR DISKECTOMY
  • NUCLEUS PULPOSUS
  • REPLACEMENT
  • SHEEP MODEL

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