Surface functionalisation of PLGA nanoparticles for gene silencing

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Surface functionalisation of PLGA nanoparticles for gene silencing. / Andersen, Morten Østergaard; Lichawska, Agata; Arpanaei, Ayyoob; Jensen, Stig Mølgaard Rask; Kaur, Harpreet; Oupicky, David; Besenbacher, Flemming; Kingshott, Peter; Kjems, Jørgen; Howard, Kenneth Alan.

In: Biomaterials, Vol. 31, No. 21, 07.2010, p. 5671-5677.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Andersen, MØ, Lichawska, A, Arpanaei, A, Jensen, SMR, Kaur, H, Oupicky, D, Besenbacher, F, Kingshott, P, Kjems, J & Howard, KA 2010, 'Surface functionalisation of PLGA nanoparticles for gene silencing', Biomaterials, vol. 31, no. 21, pp. 5671-5677. https://doi.org/10.1016/j.biomaterials.2010.03.069

APA

Andersen, M. Ø., Lichawska, A., Arpanaei, A., Jensen, S. M. R., Kaur, H., Oupicky, D., ... Howard, K. A. (2010). Surface functionalisation of PLGA nanoparticles for gene silencing. Biomaterials, 31(21), 5671-5677. https://doi.org/10.1016/j.biomaterials.2010.03.069

CBE

Andersen MØ, Lichawska A, Arpanaei A, Jensen SMR, Kaur H, Oupicky D, Besenbacher F, Kingshott P, Kjems J, Howard KA. 2010. Surface functionalisation of PLGA nanoparticles for gene silencing. Biomaterials. 31(21):5671-5677. https://doi.org/10.1016/j.biomaterials.2010.03.069

MLA

Vancouver

Andersen MØ, Lichawska A, Arpanaei A, Jensen SMR, Kaur H, Oupicky D et al. Surface functionalisation of PLGA nanoparticles for gene silencing. Biomaterials. 2010 Jul;31(21):5671-5677. https://doi.org/10.1016/j.biomaterials.2010.03.069

Author

Andersen, Morten Østergaard ; Lichawska, Agata ; Arpanaei, Ayyoob ; Jensen, Stig Mølgaard Rask ; Kaur, Harpreet ; Oupicky, David ; Besenbacher, Flemming ; Kingshott, Peter ; Kjems, Jørgen ; Howard, Kenneth Alan. / Surface functionalisation of PLGA nanoparticles for gene silencing. In: Biomaterials. 2010 ; Vol. 31, No. 21. pp. 5671-5677.

Bibtex

@article{68eb2f29baab4ba7b142c08a95c5d068,
title = "Surface functionalisation of PLGA nanoparticles for gene silencing",
abstract = "This work presents a method for decorating the surface of poly (lactide-co-glycolide) (PLGA) nanoparticles with polyethyleneimine (PEI) utilising a cetyl derivative to improve surface functionalisation and siRNA delivery. Sub-micron particles were produced by an emulsion-diffusion method using benzyl alcohol. We demonstrate by x-ray photoelectron spectroscopy (XPS), 2.6 times higher surface presentation of amines using the cetyl derivative compared to non-cetylated-PEI formulations (6.5 and 2.5{\%} surface nitrogen, respectively). The modified particles were shown by spectroscopy, fluorescent microscopy and flow cytometry to bind and mediate siRNA delivery into the human osteosarcoma cell line U2OS and the murine macrophage cell line J774.1. Specific reduction in the anti-apoptotic oncogene BCL-w in U2OS cells was achieved with particles containing cetylated-PEI (53{\%}) with no cellular toxicity. In addition, particles containing cetylated-PEI achieved 64{\%} silencing of TNFα in J774.1 cells. This rapid method for surface modification of PLGA nanoparticles promotes its application for alternative cetylated functional derivatives as a strategy to control specific biological properties of nanoparticles.",
keywords = "RNAi, siRNA, PLGA, Cetylated PEI, Drug delivery, Surface modification",
author = "Andersen, {Morten {\O}stergaard} and Agata Lichawska and Ayyoob Arpanaei and Jensen, {Stig M{\o}lgaard Rask} and Harpreet Kaur and David Oupicky and Flemming Besenbacher and Peter Kingshott and J{\o}rgen Kjems and Howard, {Kenneth Alan}",
year = "2010",
month = "7",
doi = "10.1016/j.biomaterials.2010.03.069",
language = "English",
volume = "31",
pages = "5671--5677",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "21",

}

RIS

TY - JOUR

T1 - Surface functionalisation of PLGA nanoparticles for gene silencing

AU - Andersen, Morten Østergaard

AU - Lichawska, Agata

AU - Arpanaei, Ayyoob

AU - Jensen, Stig Mølgaard Rask

AU - Kaur, Harpreet

AU - Oupicky, David

AU - Besenbacher, Flemming

AU - Kingshott, Peter

AU - Kjems, Jørgen

AU - Howard, Kenneth Alan

PY - 2010/7

Y1 - 2010/7

N2 - This work presents a method for decorating the surface of poly (lactide-co-glycolide) (PLGA) nanoparticles with polyethyleneimine (PEI) utilising a cetyl derivative to improve surface functionalisation and siRNA delivery. Sub-micron particles were produced by an emulsion-diffusion method using benzyl alcohol. We demonstrate by x-ray photoelectron spectroscopy (XPS), 2.6 times higher surface presentation of amines using the cetyl derivative compared to non-cetylated-PEI formulations (6.5 and 2.5% surface nitrogen, respectively). The modified particles were shown by spectroscopy, fluorescent microscopy and flow cytometry to bind and mediate siRNA delivery into the human osteosarcoma cell line U2OS and the murine macrophage cell line J774.1. Specific reduction in the anti-apoptotic oncogene BCL-w in U2OS cells was achieved with particles containing cetylated-PEI (53%) with no cellular toxicity. In addition, particles containing cetylated-PEI achieved 64% silencing of TNFα in J774.1 cells. This rapid method for surface modification of PLGA nanoparticles promotes its application for alternative cetylated functional derivatives as a strategy to control specific biological properties of nanoparticles.

AB - This work presents a method for decorating the surface of poly (lactide-co-glycolide) (PLGA) nanoparticles with polyethyleneimine (PEI) utilising a cetyl derivative to improve surface functionalisation and siRNA delivery. Sub-micron particles were produced by an emulsion-diffusion method using benzyl alcohol. We demonstrate by x-ray photoelectron spectroscopy (XPS), 2.6 times higher surface presentation of amines using the cetyl derivative compared to non-cetylated-PEI formulations (6.5 and 2.5% surface nitrogen, respectively). The modified particles were shown by spectroscopy, fluorescent microscopy and flow cytometry to bind and mediate siRNA delivery into the human osteosarcoma cell line U2OS and the murine macrophage cell line J774.1. Specific reduction in the anti-apoptotic oncogene BCL-w in U2OS cells was achieved with particles containing cetylated-PEI (53%) with no cellular toxicity. In addition, particles containing cetylated-PEI achieved 64% silencing of TNFα in J774.1 cells. This rapid method for surface modification of PLGA nanoparticles promotes its application for alternative cetylated functional derivatives as a strategy to control specific biological properties of nanoparticles.

KW - RNAi

KW - siRNA

KW - PLGA

KW - Cetylated PEI

KW - Drug delivery

KW - Surface modification

U2 - 10.1016/j.biomaterials.2010.03.069

DO - 10.1016/j.biomaterials.2010.03.069

M3 - Journal article

VL - 31

SP - 5671

EP - 5677

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 21

ER -