Superparamagnetic iron oxide polyacrylic acid coated γ-Fe2O3 nanoparticles do not affect kidney function but cause acute effect on the cardiovascular function in healthy mice

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • N.K. Iversen
  • S. Frische
  • Karen Thomsen, Denmark
  • C. Laustsen
  • Michael Pedersen, Aarhus University
  • ,
  • P.B.L. Hansen, University of Southern Denmark
  • ,
  • P. Bie, University of Southern Denmark
  • ,
  • J. Fresnais, UMR 7195 CNRS-UPMC-ESPCI
  • ,
  • J.-F. Berret, Bâtiment Condorcet
  • ,
  • E. Baatrup
  • T. Wang
This study describes the distribution of intravenously injected polyacrylic acid (PAA) coated γ-FeO NPs (10mgkg) at the organ, cellular and subcellular levels in healthy BALB/cJ mice and in parallel addresses the effects of NP injection on kidney function, blood pressure and vascular contractility. Magnetic resonance imaging (MRI) and transmission electron microscopy (TEM) showed accumulation of NPs in the liver within 1h after intravenous infusion, accommodated by intracellular uptake in endothelial and Kupffer cells with subsequent intracellular uptake in renal cells, particularly the cytoplasm of the proximal tubule, in podocytes and mesangial cells. The renofunctional effects of NPs were evaluated by arterial acid-base status and measurements of glomerular filtration rate (GFR) after instrumentation with chronically indwelling catheters. Arterial pH was 7.46±0.02 and 7.41±0.02 in mice 0.5h after injections of saline or NP, and did not change over the next 12h. In addition, the injections of NP did not affect arterial PCO or [HCO] either. Twenty-four and 96h after NP injections, the GFR averaged 0.35±0.04 and 0.35±0.01mlming, respectively, values which were statistically comparable with controls (0.29±0.02 and 0.33±0.1ml min 25g). Mean arterial blood pressure (MAP) decreased 12-24h after NP injections (111.1±11.5 vs 123.0±6.1min) associated with a decreased contractility of small mesenteric arteries revealed by myography to characterize endothelial function. In conclusion, our study demonstrates that accumulation of superparamagnetic iron oxide nanoparticles does not affect kidney function in healthy mice but temporarily decreases blood pressure.
Original languageEnglish
JournalToxicology and Applied Pharmacology
Pages (from-to)276-88
Number of pages13
Publication statusPublished - 15 Jan 2013

    Research areas

  • Acrylic Resins, Animals, Blood Pressure, Female, Ferric Compounds, Glomerular Filtration Rate, Hydrogen-Ion Concentration, Injections, Intravenous, Kidney, Magnetic Resonance Imaging, Magnetite Nanoparticles, Male, Mesenteric Arteries, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Muscle Contraction, Myography, Time Factors, Tissue Distribution

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