TY - JOUR
T1 - Sugar transporter proteins in Capsicum
T2 - identification, characterization, evolution and expression patterns
AU - Wei, Huawei
AU - Liu, Jia
AU - Zheng, Jiaqiu
AU - Zhou, Rong
AU - Cheng, Yuan
AU - Ruan, Meiying
AU - Ye, Qingjing
AU - Wang, Rongqing
AU - Yao, Zhuping
AU - Zhou, Guozhi
AU - Deng, Minghua
AU - Chen, Yougen
AU - Wan, Hongjian
PY - 2020
Y1 - 2020
N2 - Sugar transporter proteins (STPs) play an important role in plant growth and development and stress resistance. Pepper (Capsicum annuum L.) is a significant economic crop and is widely cultivated worldwide. However, the evolution and the roles of STP genes in the growth and development and in coping with abiotic stresses in pepper are poorly known. Here, we characterized STP gene family in pepper through integration of chromosomal location, gene structure, conserved motif, phylogeny, expression patterns in different tissues and abiotic stresses. Using bioinformatics-based methods, we identified 17 putative STP genes in pepper. Chromosome mapping indicated that they are unevenly distributed on 9 chromosomes and there are tandem duplication events. Gene structure analysis revealed that intron numbers among these CaSTP genes ranged from 2 to 5, except that CaSTP12 and CaSTP13 genes contained no introns. The phylogenetic tree of STP genes from pepper and other plant species revealed that STP genes were grouped into 4 subfamilies, suggesting that these gene subfamilies may have diverged from a single common ancestor prior to the mono-dicot split. Gene expression analysis based on RNA-seq data showed that three genes were expressed constitutively in all the tissues analyzed, implying that these genes might have specific function in pepper. Stress data analysis showed that the expression profiles of some members of the CaSTP gene family were altered under cold, heat, salt and hormone stress conditions. The present results will provide valuable information into the evolutionary relationships and functional divergence of the STP family in the future.
AB - Sugar transporter proteins (STPs) play an important role in plant growth and development and stress resistance. Pepper (Capsicum annuum L.) is a significant economic crop and is widely cultivated worldwide. However, the evolution and the roles of STP genes in the growth and development and in coping with abiotic stresses in pepper are poorly known. Here, we characterized STP gene family in pepper through integration of chromosomal location, gene structure, conserved motif, phylogeny, expression patterns in different tissues and abiotic stresses. Using bioinformatics-based methods, we identified 17 putative STP genes in pepper. Chromosome mapping indicated that they are unevenly distributed on 9 chromosomes and there are tandem duplication events. Gene structure analysis revealed that intron numbers among these CaSTP genes ranged from 2 to 5, except that CaSTP12 and CaSTP13 genes contained no introns. The phylogenetic tree of STP genes from pepper and other plant species revealed that STP genes were grouped into 4 subfamilies, suggesting that these gene subfamilies may have diverged from a single common ancestor prior to the mono-dicot split. Gene expression analysis based on RNA-seq data showed that three genes were expressed constitutively in all the tissues analyzed, implying that these genes might have specific function in pepper. Stress data analysis showed that the expression profiles of some members of the CaSTP gene family were altered under cold, heat, salt and hormone stress conditions. The present results will provide valuable information into the evolutionary relationships and functional divergence of the STP family in the future.
KW - abiotic stress
KW - Chromosome mapping
KW - gene structure
KW - phylogenetic relationship
UR - http://www.scopus.com/inward/record.url?scp=85083551580&partnerID=8YFLogxK
U2 - 10.1080/13102818.2020.1749529
DO - 10.1080/13102818.2020.1749529
M3 - Journal article
AN - SCOPUS:85083551580
SN - 1310-2818
VL - 34
SP - 341
EP - 353
JO - Biotechnology & Biotechnological Equipment
JF - Biotechnology & Biotechnological Equipment
IS - 1
ER -