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Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

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  • Interdisciplinary Nanoscience Center
  • Department of Chemistry
  • Department of Molecular Biology

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

Original languageEnglish
JournalScience
Volume313
Pages (from-to)1968-1972
Number of pages5
ISSN0036-8075
Publication statusPublished - 2006

    Research areas

  • splicing, mRNA metabolism, DEAD-box

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