Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

Christian Brix Folsted Andersen, Lionel Ballut, Jesper Sanderhoff Johansen, H. Chamieh, Klaus Hvid Nielsen, Cristiano Oliveira, Jan Skov Pedersen, B. Seraphin, H. LeHir, Gregers Rom Andersen

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

    Abstract

    In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

    Original languageEnglish
    JournalScience
    Volume313
    Pages (from-to)1968-1972
    Number of pages5
    ISSN0036-8075
    Publication statusPublished - 2006

    Keywords

    • splicing
    • mRNA metabolism
    • DEAD-box

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