Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae

Sandra Muschiol*, Simon Erlendsson, Marie-Stephanie Aschtgen, Vitor Oliveira, Peter Schmieder, Casper de Lichtenberg, Kaare Teilum, Thomas Boesen, Umit Akbey, Birgitta Henriques-Normark

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Abstract

Type IV pili are important virulence factors on the surface of many pathogenic bacteria and have been implicated in a wide range of diverse functions, including attachment, twitching motility, biofilm formation, and horizontal gene transfer. The respiratory pathogen Streptococcus pneumoniae deploys type IV pili to take up DNA during transformation. These competence pili are composed of the major pilin protein ComGC and exclusively assembled during bacterial competence, but their biogenesis remains unclear. Here, we report the high resolution NMR structure of N-terminal truncated ComGC revealing a highly flexible and structurally divergent type IV pilin. It consists of only three -helical segments forming a well-defined electronegative cavity and confined electronegative and hydrophobic patches. The structure is particularly flexible between the first and second -helix with the first helical part exhibiting slightly slower dynamics than the rest of the pilin, suggesting that the first helix is involved in forming the pilus structure core and that parts of helices two and three are primarily surface-exposed. Taken together, our results provide the first structure of a type IV pilin protein involved in the formation of competence-induced pili in Gram-positive bacteria and corroborate the remarkable structural diversity among type IV pilin proteins.

Original languageEnglish
JournalJournal of Biological Chemistry
Volume292
Issue34
Pages (from-to)14134-14146
Number of pages13
ISSN0021-9258
DOIs
Publication statusPublished - 25 Aug 2017

Keywords

  • microbiology
  • nuclear magnetic resonance (NMR)
  • protein structure
  • transformation
  • type IV pili
  • Streptococcus pneumoniae
  • horizontal gene transfer
  • major pilin
  • pneumococci
  • RESIDUAL DIPOLAR COUPLINGS
  • IV PILUS
  • NATURAL TRANSFORMATION
  • NEISSERIA-GONORRHOEAE
  • ELECTRON-MICROSCOPY
  • TWITCHING MOTILITY
  • ESCHERICHIA-COLI
  • VIBRIO-CHOLERAE
  • NMR RELAXATION
  • DNA-BINDING

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