Structure of branching enzyme- and amylomaltase modified starch produced from well-defined amylose to amylopectin substrates

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Waraporn Sorndech, University of Copenhagen
  • ,
  • Domenico Sagnelli, Department of Plant and Environmental Sciences, KU, University of Copenhagen
  • ,
  • Sebastian Meier, Technical University of Denmark
  • ,
  • Anita M. Jansson, Carlsberg Research Laboratory
  • ,
  • Byung Hoo Lee, Gachon University
  • ,
  • Bruce R. Hamaker, Purdue University
  • ,
  • Agnès Rolland-Sabaté, INRA Nantes
  • ,
  • Kim H. Hebelstrup
  • Sunanta Tongta, Suranaree University of Technology
  • ,
  • Andreas Blennow, University of Copenhagen

Thermostable branching enzyme (BE, EC from Rhodothermus obamensis in combination with amylomaltase (AM, EC from Thermus thermophilus was used to modify starch structure exploring potentials to extensively increase the number of branch points in starch. Amylose is an important constituent in starch and the effect of amylose on enzyme catalysis was investigated using amylose-only barley starch (AO) and waxy maize starch (WX) in well-defined ratios. All products were analysed for amylopectin chain length distribution, α-1,6 glucosidic linkages content, molar mass distribution and digestibility by using rat intestinal α-glucosidases. For each enzyme treatment series, increased AO content resulted in a higher rate of α-1,6 glucosidic linkage formation but as an effect of the very low initial branching of the AO, the final content of α-1,6 glucosidic linkages was slightly lower as compared to the high amylopectin substrates. However, an increase specifically in short chains was produced at high AO levels. The molar mass distribution for the enzyme treated samples was lower as compared with substrate WX and AO, indicating the presence of hydrolytic activity as well as cyclisation of the substrate. For all samples, increased amylose substrate showed decreased α- and β-amylolysis. Surprisingly, hydrolysis with rat intestinal α-glucosidases was higher with increasing α-1,6 glucosidic linkage content and decreasing M¯w indicating that steric hindrance towards the α-glucosidases was directed by the molar mass rather that the branching density of the glucan per se. Our data demonstrate that a higher amylose content in the substrate starch efficiently produces α-1,6 glucosidic linkages and that the present of amylose generates a higher M¯w and more resistant product than amylopectin. The combination of BE → AM → BE provided somewhat more resistant α-glucan products as compared to BE alone.

Original languageEnglish
JournalCarbohydrate Polymers
Pages (from-to)51-61
Number of pages11
Publication statusPublished - 5 Nov 2016

    Research areas

  • Amylomaltase, Amylose content, Branching enzyme, Starch degradation

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