Structural signatures in EPR3 define a unique class of plant carbohydrate receptors

Jaslyn E.M.M. Wong; Kira Gysel; Thea G. Birkefeldt; Maria Vinther; Artur Muszyński; Parastoo Azadi; Nick S. Laursen; John T. Sullivan; Clive W. Ronson; Jens Stougaard; Kasper R. Andersen

doi:10.1038/s41467-020-17568-9

Structural signatures in EPR3 define a unique class of plant carbohydrate receptors

Jaslyn E.M.M. Wong, Kira Gysel, Thea G. Birkefeldt, Maria Vinther, Artur Muszyński, Parastoo Azadi, Nick S. Laursen, John T. Sullivan, Clive W. Ronson, Jens Stougaard, Kasper R. Andersen^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

37 Citations (Scopus)

Abstract

Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.

Original language	English
Article number	3797
Journal	Nature Communications
Volume	11
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-020-17568-9
Publication status	Published - Jul 2020

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title = "Structural signatures in EPR3 define a unique class of plant carbohydrate receptors",

abstract = "Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.",

author = "Wong, {Jaslyn E.M.M.} and Kira Gysel and Birkefeldt, {Thea G.} and Maria Vinther and Artur Muszy{\'n}ski and Parastoo Azadi and Laursen, {Nick S.} and Sullivan, {John T.} and Ronson, {Clive W.} and Jens Stougaard and Andersen, {Kasper R.}",

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AU - Wong, Jaslyn E.M.M.

AU - Gysel, Kira

AU - Birkefeldt, Thea G.

AU - Vinther, Maria

AU - Muszyński, Artur

AU - Azadi, Parastoo

AU - Laursen, Nick S.

AU - Sullivan, John T.

AU - Ronson, Clive W.

AU - Stougaard, Jens

AU - Andersen, Kasper R.

PY - 2020/7

Y1 - 2020/7

N2 - Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.

AB - Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.

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Structural signatures in EPR3 define a unique class of plant carbohydrate receptors

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