Stress and corticosterone increase the readily releasable pool of glutamate vesicles in synaptic terminals of prefrontal and frontal cortex

TY - JOUR

T1 - Stress and corticosterone increase the readily releasable pool of glutamate vesicles in synaptic terminals of prefrontal and frontal cortex

AU - Treccani, G

AU - Musazzi, L

AU - Perego, C

AU - Milanese, M

AU - Nava, N

AU - Bonifacino, T

AU - Lamanna, J

AU - Malgaroli, A

AU - Drago, F

AU - Racagni, G

AU - Nyengaard, J R

AU - Wegener, Gregers

AU - Bonanno, G

AU - Popoli, M

PY - 2014/4

Y1 - 2014/4

N2 - Stress and glucocorticoids alter glutamatergic transmission, and the outcome of stress may range from plasticity enhancing effects to noxious, maladaptive changes. We have previously demonstrated that acute stress rapidly increases glutamate release in prefrontal and frontal cortex via glucocorticoid receptor and accumulation of presynaptic SNARE complex. Here we compared the ex vivo effects of acute stress on glutamate release with those of in vitro application of corticosterone, to analyze whether acute effect of stress on glutamatergic transmission is mediated by local synaptic action of corticosterone. We found that acute stress increases both the readily releasable pool (RRP) of vesicles and depolarization-evoked glutamate release, while application in vitro of corticosterone rapidly increases the RRP, an effect dependent on synaptic receptors for the hormone, but does not induce glutamate release for up to 20 min. These findings indicate that corticosterone mediates the enhancement of glutamate release induced by acute stress, and the rapid non-genomic action of the hormone is necessary but not sufficient for this effect.Molecular Psychiatry advance online publication, 18 February 2014; doi:10.1038/mp.2014.5.

AB - Stress and glucocorticoids alter glutamatergic transmission, and the outcome of stress may range from plasticity enhancing effects to noxious, maladaptive changes. We have previously demonstrated that acute stress rapidly increases glutamate release in prefrontal and frontal cortex via glucocorticoid receptor and accumulation of presynaptic SNARE complex. Here we compared the ex vivo effects of acute stress on glutamate release with those of in vitro application of corticosterone, to analyze whether acute effect of stress on glutamatergic transmission is mediated by local synaptic action of corticosterone. We found that acute stress increases both the readily releasable pool (RRP) of vesicles and depolarization-evoked glutamate release, while application in vitro of corticosterone rapidly increases the RRP, an effect dependent on synaptic receptors for the hormone, but does not induce glutamate release for up to 20 min. These findings indicate that corticosterone mediates the enhancement of glutamate release induced by acute stress, and the rapid non-genomic action of the hormone is necessary but not sufficient for this effect.Molecular Psychiatry advance online publication, 18 February 2014; doi:10.1038/mp.2014.5.

U2 - 10.1038/mp.2014.5

DO - 10.1038/mp.2014.5

M3 - Journal article

C2 - 24535456

SN - 1359-4184

VL - 19

SP - 433

EP - 443

JO - Molecular Psychiatry

JF - Molecular Psychiatry

ER -