Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples? / Otte, Julia M.; Blackwell, Nia; Soos, Viktoria; Rughöft, Saskia; Maisch, Markus; Kappler, Andreas; Kleindienst, Sara; Schmidt, Caroline.

In: FEMS Microbiology Ecology, Vol. 94, No. 12, fiy189, 01.12.2018.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Otte, JM, Blackwell, N, Soos, V, Rughöft, S, Maisch, M, Kappler, A, Kleindienst, S & Schmidt, C 2018, 'Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?', FEMS Microbiology Ecology, vol. 94, no. 12, fiy189. https://doi.org/10.1093/femsec/fiy189

APA

Otte, J. M., Blackwell, N., Soos, V., Rughöft, S., Maisch, M., Kappler, A., Kleindienst, S., & Schmidt, C. (2018). Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples? FEMS Microbiology Ecology, 94(12), [fiy189]. https://doi.org/10.1093/femsec/fiy189

CBE

Otte JM, Blackwell N, Soos V, Rughöft S, Maisch M, Kappler A, Kleindienst S, Schmidt C. 2018. Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?. FEMS Microbiology Ecology. 94(12):Article fiy189. https://doi.org/10.1093/femsec/fiy189

MLA

Vancouver

Otte JM, Blackwell N, Soos V, Rughöft S, Maisch M, Kappler A et al. Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples? FEMS Microbiology Ecology. 2018 Dec 1;94(12). fiy189. https://doi.org/10.1093/femsec/fiy189

Author

Otte, Julia M. ; Blackwell, Nia ; Soos, Viktoria ; Rughöft, Saskia ; Maisch, Markus ; Kappler, Andreas ; Kleindienst, Sara ; Schmidt, Caroline. / Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?. In: FEMS Microbiology Ecology. 2018 ; Vol. 94, No. 12.

Bibtex

@article{f8f6079c3e5e40bea01264909a9d0aec,
title = "Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?",
abstract = "To distinguish between biotic and abiotic processes in laboratory experiments with environmental samples, an effective sterilization method is required that prevents biological activity but does not change physico-geochemical properties of samples. We compared standard sterilization methods with respect to their impact on microbial abundance and activity. We exposed marine sediment to (i) autoclaving, (ii) gamma-radiation or (iii) sodium azide (NaN3) and determined how nucleic acids, microbial productivity, colony forming units (CFUs) and community composition of microorganisms, fungi, unicellular protists and protozoa were affected. In autoclaved and gamma-sterilized sediments, only few colonies formed within 16 days. After addition of NaN3 to the sediment, numerous CFUs ( > 50) but lower 3H-leucine incorporation rates, i.e. lower protein biosynthesis rates, were found compared to the other two sterilization techniques. Extractable RNA was detected immediately after all sterilization treatments (0.2-17.9 ng/g dry sediment) but decreased substantially by 84%-98% after 16 days of incubation. The total organic carbon content increased from 18 mg L-1 to 220 mg L-1 (autoclaving) and 150 mg L-1 (gamma-radiation) after sterilization. We compare advantages and disadvantages for each tested sterilization method and provide a helpful decision-making resource for choosing the appropriate sterilization technique for environmental studies, particularly for marine sediments.",
keywords = "H-leucine, Autoclaving, Gamma-radiation, M{\"o}ssbauer spectroscopy, RNA, Sodium azide, T-RFLP",
author = "Otte, {Julia M.} and Nia Blackwell and Viktoria Soos and Saskia Rugh{\"o}ft and Markus Maisch and Andreas Kappler and Sara Kleindienst and Caroline Schmidt",
year = "2018",
month = dec,
day = "1",
doi = "10.1093/femsec/fiy189",
language = "English",
volume = "94",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "12",

}

RIS

TY - JOUR

T1 - Sterilization impacts on marine sediment-Are we able to inactivate microorganisms in environmental samples?

AU - Otte, Julia M.

AU - Blackwell, Nia

AU - Soos, Viktoria

AU - Rughöft, Saskia

AU - Maisch, Markus

AU - Kappler, Andreas

AU - Kleindienst, Sara

AU - Schmidt, Caroline

PY - 2018/12/1

Y1 - 2018/12/1

N2 - To distinguish between biotic and abiotic processes in laboratory experiments with environmental samples, an effective sterilization method is required that prevents biological activity but does not change physico-geochemical properties of samples. We compared standard sterilization methods with respect to their impact on microbial abundance and activity. We exposed marine sediment to (i) autoclaving, (ii) gamma-radiation or (iii) sodium azide (NaN3) and determined how nucleic acids, microbial productivity, colony forming units (CFUs) and community composition of microorganisms, fungi, unicellular protists and protozoa were affected. In autoclaved and gamma-sterilized sediments, only few colonies formed within 16 days. After addition of NaN3 to the sediment, numerous CFUs ( > 50) but lower 3H-leucine incorporation rates, i.e. lower protein biosynthesis rates, were found compared to the other two sterilization techniques. Extractable RNA was detected immediately after all sterilization treatments (0.2-17.9 ng/g dry sediment) but decreased substantially by 84%-98% after 16 days of incubation. The total organic carbon content increased from 18 mg L-1 to 220 mg L-1 (autoclaving) and 150 mg L-1 (gamma-radiation) after sterilization. We compare advantages and disadvantages for each tested sterilization method and provide a helpful decision-making resource for choosing the appropriate sterilization technique for environmental studies, particularly for marine sediments.

AB - To distinguish between biotic and abiotic processes in laboratory experiments with environmental samples, an effective sterilization method is required that prevents biological activity but does not change physico-geochemical properties of samples. We compared standard sterilization methods with respect to their impact on microbial abundance and activity. We exposed marine sediment to (i) autoclaving, (ii) gamma-radiation or (iii) sodium azide (NaN3) and determined how nucleic acids, microbial productivity, colony forming units (CFUs) and community composition of microorganisms, fungi, unicellular protists and protozoa were affected. In autoclaved and gamma-sterilized sediments, only few colonies formed within 16 days. After addition of NaN3 to the sediment, numerous CFUs ( > 50) but lower 3H-leucine incorporation rates, i.e. lower protein biosynthesis rates, were found compared to the other two sterilization techniques. Extractable RNA was detected immediately after all sterilization treatments (0.2-17.9 ng/g dry sediment) but decreased substantially by 84%-98% after 16 days of incubation. The total organic carbon content increased from 18 mg L-1 to 220 mg L-1 (autoclaving) and 150 mg L-1 (gamma-radiation) after sterilization. We compare advantages and disadvantages for each tested sterilization method and provide a helpful decision-making resource for choosing the appropriate sterilization technique for environmental studies, particularly for marine sediments.

KW - H-leucine

KW - Autoclaving

KW - Gamma-radiation

KW - Mössbauer spectroscopy

KW - RNA

KW - Sodium azide

KW - T-RFLP

UR - http://www.scopus.com/inward/record.url?scp=85055072674&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiy189

DO - 10.1093/femsec/fiy189

M3 - Journal article

C2 - 30247566

AN - SCOPUS:85055072674

VL - 94

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 12

M1 - fiy189

ER -