Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin. / Ghorbani, Sadegh; Eyni, Hossein; Khosrowpour, Zahra; Asl, Leila Salari; Shabani, Ronak; Nazari, Hojjatollah; Mehdizadeh, Mehdi; Warkiani, Majid Ebrahimi; Amjadi, FatemehSadat.

In: Polymers for Advanced Technologies, Vol. 30, No. 12, 12.2019, p. 3011-3025.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Ghorbani, S, Eyni, H, Khosrowpour, Z, Asl, LS, Shabani, R, Nazari, H, Mehdizadeh, M, Warkiani, ME & Amjadi, F 2019, 'Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin', Polymers for Advanced Technologies, vol. 30, no. 12, pp. 3011-3025. https://doi.org/10.1002/pat.4733

APA

Ghorbani, S., Eyni, H., Khosrowpour, Z., Asl, L. S., Shabani, R., Nazari, H., ... Amjadi, F. (2019). Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin. Polymers for Advanced Technologies, 30(12), 3011-3025. https://doi.org/10.1002/pat.4733

CBE

Ghorbani S, Eyni H, Khosrowpour Z, Asl LS, Shabani R, Nazari H, Mehdizadeh M, Warkiani ME, Amjadi F. 2019. Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin. Polymers for Advanced Technologies. 30(12):3011-3025. https://doi.org/10.1002/pat.4733

MLA

Vancouver

Author

Ghorbani, Sadegh ; Eyni, Hossein ; Khosrowpour, Zahra ; Asl, Leila Salari ; Shabani, Ronak ; Nazari, Hojjatollah ; Mehdizadeh, Mehdi ; Warkiani, Majid Ebrahimi ; Amjadi, FatemehSadat. / Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin. In: Polymers for Advanced Technologies. 2019 ; Vol. 30, No. 12. pp. 3011-3025.

Bibtex

@article{f89d3598a84f46b79b763e32492bcee4,
title = "Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin",
abstract = "Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi-walled carbon nanotubes (MWCNTs) were incorporated into poly(l-lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse-transcription polymerase chain reaction (RT-PCR), and qRT-PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C-kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.",
keywords = "antioxidants, in vitro spermatogenesis, naringenin, PLLA, MWCNTs scaffolds, spermatogonial stem cells (SSCs), OXIDATIVE STRESS, IN-VITRO, TESTICULAR FUNCTION, MICE, DIFFERENTIATION, TRANSPLANTATION, PROLIFERATION, FERTILITY, EFFICACY, CULTURE, PLLA/MWCNTs scaffolds",
author = "Sadegh Ghorbani and Hossein Eyni and Zahra Khosrowpour and Asl, {Leila Salari} and Ronak Shabani and Hojjatollah Nazari and Mehdi Mehdizadeh and Warkiani, {Majid Ebrahimi} and FatemehSadat Amjadi",
year = "2019",
month = "12",
doi = "10.1002/pat.4733",
language = "English",
volume = "30",
pages = "3011--3025",
journal = "Polymers for Advanced Technologies",
issn = "1042-7147",
publisher = "JohnWiley & Sons Ltd.",
number = "12",

}

RIS

TY - JOUR

T1 - Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin

AU - Ghorbani, Sadegh

AU - Eyni, Hossein

AU - Khosrowpour, Zahra

AU - Asl, Leila Salari

AU - Shabani, Ronak

AU - Nazari, Hojjatollah

AU - Mehdizadeh, Mehdi

AU - Warkiani, Majid Ebrahimi

AU - Amjadi, FatemehSadat

PY - 2019/12

Y1 - 2019/12

N2 - Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi-walled carbon nanotubes (MWCNTs) were incorporated into poly(l-lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse-transcription polymerase chain reaction (RT-PCR), and qRT-PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C-kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.

AB - Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi-walled carbon nanotubes (MWCNTs) were incorporated into poly(l-lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse-transcription polymerase chain reaction (RT-PCR), and qRT-PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C-kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.

KW - antioxidants

KW - in vitro spermatogenesis

KW - naringenin

KW - PLLA

KW - MWCNTs scaffolds

KW - spermatogonial stem cells (SSCs)

KW - OXIDATIVE STRESS

KW - IN-VITRO

KW - TESTICULAR FUNCTION

KW - MICE

KW - DIFFERENTIATION

KW - TRANSPLANTATION

KW - PROLIFERATION

KW - FERTILITY

KW - EFFICACY

KW - CULTURE

KW - PLLA/MWCNTs scaffolds

UR - http://www.scopus.com/inward/record.url?scp=85070073132&partnerID=8YFLogxK

U2 - 10.1002/pat.4733

DO - 10.1002/pat.4733

M3 - Journal article

VL - 30

SP - 3011

EP - 3025

JO - Polymers for Advanced Technologies

JF - Polymers for Advanced Technologies

SN - 1042-7147

IS - 12

ER -