Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l-lactic acid)/multi-walled carbon nanotube scaffolds and naringenin

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DOI

  • Sadegh Ghorbani
  • Hossein Eyni, Tarbiat Modares Univ, Tarbiat Modares University, Sch Med Sci, Dept Anat Sci
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  • Zahra Khosrowpour, Tarbiat Modares Univ, Tarbiat Modares University, Sch Med Sci, Dept Anat Sci
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  • Leila Salari Asl, Tarbiat Modares Univ, Tarbiat Modares University, Sch Med Sci, Dept Anat Sci
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  • Ronak Shabani, Iran Univ Med Sci, Iran University of Medical Sciences, Sch Med, Dept Anat Sci
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  • Hojjatollah Nazari, Tarbiat Modares Univ, Tarbiat Modares University, Fac Med Sci, Dept Cell Therapy & Hematol
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  • Mehdi Mehdizadeh, Iran Univ Med Sci, Iran University of Medical Sciences, Sch Med, Dept Anat Sci, University of Technology Sydney, New South Wales
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  • Majid Ebrahimi Warkiani, Sechenov First Moscow State Univ, Sechenov First Moscow State Medical University, Inst Mol Med
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  • FatemehSadat Amjadi, Iran Univ Med Sci, Iran University of Medical Sciences, Sch Med, Dept Anat Sci

Spermatogenesis is a process in which animals generate spermatozoa from spermatogonial stem cells (SSCs). Successful in vitro differentiation of SSCs towards spermatids holds a significant promise for regeneration of impaired spermatogenesis. The present study aims to evaluate the efficiency of a 3D culture containing naringenin on proliferation and differentiation potentials of mouse SSCs. In this study, multi-walled carbon nanotubes (MWCNTs) were incorporated into poly(l-lactic acid) (PLLA) fibers via electrospinning technique. The fibrous PLLA/MWCNTs were studied by Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), water contact angle measurements, electrical conductivity, and mechanical properties. Next, the SSCs were seeded into the PLLA/MWCNTs scaffolds and exhibited preferable survival and differentiation efficiency to subsequent cell lines. To shed more light on this matter, the immunocytochemistry, reverse-transcription polymerase chain reaction (RT-PCR), and qRT-PCR results showed that the aforementioned cells on the 3D fabrics overexpressed the C-kit and SYCP3 proteins. In addition, the reactive oxygen species (ROS) measurement data demonstrated that naringenin, an effective antioxidant, plays an important role in in vitro spermatogenesis. Taken together, the results of this study revealed the synergistic effects of 3D scaffolds and naringenin for efficient spermatogenesis in laboratories.

Original languageEnglish
JournalPolymers for Advanced Technologies
Volume30
Issue12
Pages (from-to)3011-3025
ISSN1042-7147
DOIs
Publication statusPublished - Dec 2019

    Research areas

  • antioxidants, in vitro spermatogenesis, naringenin, PLLA, MWCNTs scaffolds, spermatogonial stem cells (SSCs), OXIDATIVE STRESS, IN-VITRO, TESTICULAR FUNCTION, MICE, DIFFERENTIATION, TRANSPLANTATION, PROLIFERATION, FERTILITY, EFFICACY, CULTURE, PLLA/MWCNTs scaffolds

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