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Specificity of binding of the low density lipoprotein receptor-related protein to different conformational states of the clade E serpins plasminogen activator inhibitor-1 and proteinase nexin-1

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  • Department of Molecular Biology
The low density lipoprotein receptor-related protein (LRP) is the principal clearance receptor for serpins and serpin-proteinase complexes. The ligand binding regions of LRP consist of clusters of cysteine-rich approximately 40-residue complement-like repeats (CR), with cluster II being the principal ligand-binding region. To better understand the specificity of binding at different sites within the cluster and the ability of LRP to discriminate in vivo between uncomplexed and proteinase-complexed serpins, we have systematically examined the affinities of plasminogen activator inhibitor-1 (PAI-1) and proteinase nexin-1 (PN-1) in their native, cleaved, and proteinase-complexed states to (CR)(2) and (CR)(3) fragments of LRP cluster II. A consistent blue shift of the CR domain tryptophan fluorescence suggested a common mode of serpin binding, involving lysines on the serpin engaging the acidic region around the calcium binding site of the CR domain. High affinity binding of non-proteinase-complexed PAI-1 and PN-1 occurred to all fragments containing three CR domains (3-59 nm) and most that contain only two CR domains, although binding energies to different (CR)(3) fragments differed by up to 18% for PAI-1 and 9% for PN-1. No detectable difference in affinity was seen between native and cleaved serpin. However, the presence of proteinase in complex with the serpin enhanced affinity modestly and presumably nonspecifically. This may be sufficient to give preferential binding of such complexes in vivo at the relevant physiological concentrations.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume284
Issue27
Pages (from-to)17989-97
Number of pages9
ISSN0021-9258
DOIs
Publication statusPublished - 3 Jul 2009

    Research areas

  • Amyloid beta-Protein Precursor, Binding Sites, Escherichia coli, Humans, LDL-Receptor Related Protein 1, Lysine, Molecular Weight, Peptide Fragments, Plasminogen Activator Inhibitor 1, Protease Nexins, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Receptors, Cell Surface, Spectrometry, Fluorescence

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