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Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

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We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries.
Original languageEnglish
JournalI E E E Engineering in Medicine and Biology Society. Conference Proceedings
Volume2012
Pages (from-to)2416-2419
Number of pages4
ISSN2375-7477
DOIs
Publication statusPublished - Aug 2012
Event34th Annual International Conference of IEEE - San Diego, United States
Duration: 28 Aug 20121 Sep 2012
Conference number: 34th

Conference

Conference34th Annual International Conference of IEEE
Number34th
CountryUnited States
CitySan Diego
Period28/08/201201/09/2012

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