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Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

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Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor. / Jepsen, Morten Leth; Harmsen, Charlotte; Godbole, Adwait Anand; Nagaraja, Valakunja; Knudsen, Birgitta R; Ho, Yi-Ping.

In: Nanoscale, Vol. 8, No. 1, 2016, p. 358-364.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Jepsen, ML, Harmsen, C, Godbole, AA, Nagaraja, V, Knudsen, BR & Ho, Y-P 2016, 'Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor', Nanoscale, vol. 8, no. 1, pp. 358-364. https://doi.org/10.1039/c5nr06326d

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Author

Jepsen, Morten Leth ; Harmsen, Charlotte ; Godbole, Adwait Anand ; Nagaraja, Valakunja ; Knudsen, Birgitta R ; Ho, Yi-Ping. / Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor. In: Nanoscale. 2016 ; Vol. 8, No. 1. pp. 358-364.

Bibtex

@article{cc3e8c5a449e4bfb9c0c4b977fd50325,
title = "Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor",
abstract = "We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.",
author = "Jepsen, {Morten Leth} and Charlotte Harmsen and Godbole, {Adwait Anand} and Valakunja Nagaraja and Knudsen, {Birgitta R} and Yi-Ping Ho",
year = "2016",
doi = "10.1039/c5nr06326d",
language = "English",
volume = "8",
pages = "358--364",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "ROYAL SOC CHEMISTRY",
number = "1",

}

RIS

TY - JOUR

T1 - Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

AU - Jepsen, Morten Leth

AU - Harmsen, Charlotte

AU - Godbole, Adwait Anand

AU - Nagaraja, Valakunja

AU - Knudsen, Birgitta R

AU - Ho, Yi-Ping

PY - 2016

Y1 - 2016

N2 - We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

AB - We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

U2 - 10.1039/c5nr06326d

DO - 10.1039/c5nr06326d

M3 - Journal article

C2 - 26616006

VL - 8

SP - 358

EP - 364

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 1

ER -