Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction

Yuya Hayashi; Teodora Miclaus; Carsten Scavenius; Katarzyna Kwiatkowska; Andrzej Sobota; Péter Engelmann; Janeck James Scott-Fordsmand; Jan Johannes Enghild; Duncan S Sutherland

doi:10.1021/es404132w

Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction

Yuya Hayashi, Teodora Miclaus, Carsten Scavenius, Katarzyna Kwiatkowska, Andrzej Sobota, Péter Engelmann, Janeck James Scott-Fordsmand, Jan Johannes Enghild, Duncan S Sutherland

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas pre-formed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes pre-formed of EfCP (p < 0.05). An EfCP-AgNP mimicry made of a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.

Original language	English
Journal	Environmental Science & Technology (Washington)
Volume	47
Issue	24
Pages (from-to)	14367-14375
Number of pages	9
ISSN	0013-936X
DOIs	https://doi.org/10.1021/es404132w
Publication status	Published - 18 Nov 2013

Access to Document

10.1021/es404132w

Cite this

Hayashi, Y., Miclaus, T., Scavenius, C., Kwiatkowska, K., Sobota, A., Engelmann, P., Scott-Fordsmand, J. J., Enghild, J. J., & Sutherland, D. S. (2013). Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction. Environmental Science & Technology (Washington), 47(24), 14367-14375. https://doi.org/10.1021/es404132w

@article{e66232980fb94207931a0578d0b7a7d1,

title = "Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction",

abstract = "Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas pre-formed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes pre-formed of EfCP (p < 0.05). An EfCP-AgNP mimicry made of a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles. ",

author = "Yuya Hayashi and Teodora Miclaus and Carsten Scavenius and Katarzyna Kwiatkowska and Andrzej Sobota and P{\'e}ter Engelmann and Scott-Fordsmand, {Janeck James} and Enghild, {Jan Johannes} and Sutherland, {Duncan S}",

year = "2013",

month = nov,

day = "18",

doi = "10.1021/es404132w",

language = "English",

volume = "47",

pages = "14367--14375",

journal = "Environmental Science & Technology (Washington)",

issn = "0013-936X",

publisher = "AMER CHEMICAL SOC",

number = "24",

}

Hayashi, Y, Miclaus, T, Scavenius, C, Kwiatkowska, K, Sobota, A, Engelmann, P, Scott-Fordsmand, JJ , Enghild, JJ & Sutherland, DS 2013, 'Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction', Environmental Science & Technology (Washington), vol. 47, no. 24, pp. 14367-14375. https://doi.org/10.1021/es404132w

Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction. / Hayashi, Yuya; Miclaus, Teodora; Scavenius, Carsten et al.
In: Environmental Science & Technology (Washington), Vol. 47, No. 24, 18.11.2013, p. 14367-14375.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Species Differences Take Shape at Nanoparticles

T2 - Protein-Corona Made of the Native Repertoire Assists Cellular Interaction

AU - Hayashi, Yuya

AU - Miclaus, Teodora

AU - Scavenius, Carsten

AU - Kwiatkowska, Katarzyna

AU - Sobota, Andrzej

AU - Engelmann, Péter

AU - Scott-Fordsmand, Janeck James

AU - Enghild, Jan Johannes

AU - Sutherland, Duncan S

PY - 2013/11/18

Y1 - 2013/11/18

N2 - Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas pre-formed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes pre-formed of EfCP (p < 0.05). An EfCP-AgNP mimicry made of a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.

AB - Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas pre-formed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes pre-formed of EfCP (p < 0.05). An EfCP-AgNP mimicry made of a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.

U2 - 10.1021/es404132w

DO - 10.1021/es404132w

M3 - Journal article

C2 - 24245550

SN - 0013-936X

VL - 47

SP - 14367

EP - 14375

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

IS - 24

ER -

Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction

Abstract

Access to Document

Fingerprint

Cite this