Species Differences Take Shape at Nanoparticles: Protein-Corona Made of the Native Repertoire Assists Cellular Interaction

Yuya Hayashi, Teodora Miclaus, Carsten Scavenius, Katarzyna Kwiatkowska, Andrzej Sobota, Péter Engelmann, Janeck James Scott-Fordsmand, Jan Johannes Enghild, Duncan S Sutherland

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Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas pre-formed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes pre-formed of EfCP (p < 0.05). An EfCP-AgNP mimicry made of a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.
Original languageEnglish
JournalEnvironmental Science & Technology (Washington)
Pages (from-to)14367-14375
Number of pages9
Publication statusPublished - 18 Nov 2013

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