Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus

Maiken Krogsbaek; Nick Yin Larsen; Anne M Landau; Connie Sanchez; Jens Randel Nyengaard

doi:10.1016/j.jchemneu.2022.102205

Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus

Maiken Krogsbaek^*, Nick Yin Larsen, Anne M Landau, Connie Sanchez, Jens Randel Nyengaard

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

4 Citations (Scopus)

5 Downloads (Pure)

Abstract

Detailed quantification of brain tissue provides a deeper understanding of changes in expression and function. We have created a pipeline to study the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus using high resolution fluorescence microscopy and receptor autoradiography. The workflow involved structured serial sampling of rat hypothalamic nuclei, in situ detection of mRNA and receptor expression, and advanced image analysis. Our results demonstrate how maintaining spatial information can lead to increased understanding of RNA and protein expression. In addition, we show the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus.

Original language	English
Article number	102205
Journal	Journal of Chemical Neuroanatomy
Volume	127
ISSN	0891-0618
DOIs	https://doi.org/10.1016/j.jchemneu.2022.102205
Publication status	Published - Jan 2023

Keywords

Autoradiography
Hypothalamus
Kappa Opioid Receptor
RNAscope
Spatial image quantification

Access to Document

10.1016/j.jchemneu.2022.102205Licence: CC BY

1-s2.0-S0891061822001351-mainFinal published version, 6.12 MBLicence: CC BY

Cite this

@article{e95220a0b6334840928fbb602bdd9547,

title = "Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus",

abstract = "Detailed quantification of brain tissue provides a deeper understanding of changes in expression and function. We have created a pipeline to study the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus using high resolution fluorescence microscopy and receptor autoradiography. The workflow involved structured serial sampling of rat hypothalamic nuclei, in situ detection of mRNA and receptor expression, and advanced image analysis. Our results demonstrate how maintaining spatial information can lead to increased understanding of RNA and protein expression. In addition, we show the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus.",

keywords = "Autoradiography, Hypothalamus, Kappa Opioid Receptor, RNAscope, Spatial image quantification",

author = "Maiken Krogsbaek and Larsen, {Nick Yin} and Landau, {Anne M} and Connie Sanchez and Nyengaard, {Jens Randel}",

year = "2023",

month = jan,

doi = "10.1016/j.jchemneu.2022.102205",

language = "English",

volume = "127",

journal = "Journal of Chemical Neuroanatomy",

issn = "0891-0618",

publisher = "Elsevier",

}

Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus. / Krogsbaek, Maiken ; Larsen, Nick Yin ; Landau, Anne M et al.
In: Journal of Chemical Neuroanatomy, Vol. 127, 102205, 01.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus

AU - Krogsbaek, Maiken

AU - Larsen, Nick Yin

AU - Landau, Anne M

AU - Sanchez, Connie

AU - Nyengaard, Jens Randel

PY - 2023/1

Y1 - 2023/1

N2 - Detailed quantification of brain tissue provides a deeper understanding of changes in expression and function. We have created a pipeline to study the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus using high resolution fluorescence microscopy and receptor autoradiography. The workflow involved structured serial sampling of rat hypothalamic nuclei, in situ detection of mRNA and receptor expression, and advanced image analysis. Our results demonstrate how maintaining spatial information can lead to increased understanding of RNA and protein expression. In addition, we show the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus.

AB - Detailed quantification of brain tissue provides a deeper understanding of changes in expression and function. We have created a pipeline to study the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus using high resolution fluorescence microscopy and receptor autoradiography. The workflow involved structured serial sampling of rat hypothalamic nuclei, in situ detection of mRNA and receptor expression, and advanced image analysis. Our results demonstrate how maintaining spatial information can lead to increased understanding of RNA and protein expression. In addition, we show the detailed expression patterns of the kappa opioid receptor in the rat hypothalamus.

KW - Autoradiography

KW - Hypothalamus

KW - Kappa Opioid Receptor

KW - RNAscope

KW - Spatial image quantification

U2 - 10.1016/j.jchemneu.2022.102205

DO - 10.1016/j.jchemneu.2022.102205

M3 - Journal article

C2 - 36464066

SN - 0891-0618

VL - 127

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

M1 - 102205

ER -

Spatial quantification of single cell mRNA and ligand binding of the kappa opioid receptor in the rat hypothalamus

Abstract

Keywords

Access to Document

Fingerprint

Cite this