Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes

Teodora Miclaus; Vladimir Bochenkov; Ryosuke Ogaki; Ken Howard; Duncan S Sutherland

doi:10.1021/nl500277c

Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes

Teodora Miclaus, Vladimir Bochenkov, Ryosuke Ogaki, Ken Howard, Duncan S Sutherland

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

88 Citations (Scopus)

Abstract

Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).

Original language	English
Journal	Nano Letters
Volume	14
Issue	4
Pages (from-to)	2086-2093
Number of pages	8
ISSN	1530-6984
DOIs	https://doi.org/10.1021/nl500277c
Publication status	Published - 11 Mar 2014

Keywords

Silver nanocubes
soft corona
hard corona
localized surface plasmon resonance
protein corona quantification

Access to Document

10.1021/nl500277c

http://pubs.acs.org/doi/abs/10.1021/nl500277c

Cite this

@article{72a6ecc286764fdd92ce06a4066b894f,

title = "Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes",

abstract = "Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).",

keywords = "Silver nanocubes, soft corona, hard corona, localized surface plasmon resonance, protein corona quantification",

author = "Teodora Miclaus and Vladimir Bochenkov and Ryosuke Ogaki and Ken Howard and Sutherland, {Duncan S}",

year = "2014",

month = mar,

day = "11",

doi = "10.1021/nl500277c",

language = "English",

volume = "14",

pages = "2086--2093",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes

AU - Miclaus, Teodora

AU - Bochenkov, Vladimir

AU - Ogaki, Ryosuke

AU - Howard, Ken

AU - Sutherland, Duncan S

PY - 2014/3/11

Y1 - 2014/3/11

N2 - Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).

AB - Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions).

KW - Silver nanocubes

KW - soft corona

KW - hard corona

KW - localized surface plasmon resonance

KW - protein corona quantification

U2 - 10.1021/nl500277c

DO - 10.1021/nl500277c

M3 - Journal article

C2 - 24617413

SN - 1530-6984

VL - 14

SP - 2086

EP - 2093

JO - Nano Letters

JF - Nano Letters

IS - 4

ER -

Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes

Abstract

Keywords

Access to Document

Fingerprint

Cite this