Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

Rajendra Kumar Gurumurthy; Naveen Kumar; Cindrilla Chumduri

doi:10.1016/j.xpro.2021.100969

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

Original language	English
Article number	100969
Journal	STAR Protocols
Volume	2
Issue	4
Number of pages	21
DOIs	https://doi.org/10.1016/j.xpro.2021.100969
Publication status	Published - 17 Nov 2021
Externally published	Yes

Keywords

Gene Expression
In Situ Hybridization
Microscopy

Access to Document

10.1016/j.xpro.2021.100969

Cite this

@article{41391b0540ec4c958ae5dec8e75497ed,

title = "Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract",

abstract = "Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).",

keywords = "Gene Expression, In Situ Hybridization, Microscopy",

author = "{Kumar Gurumurthy}, Rajendra and Naveen Kumar and Cindrilla Chumduri",

year = "2021",

month = nov,

day = "17",

doi = "10.1016/j.xpro.2021.100969",

language = "English",

volume = "2",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "4",

}

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract. / Kumar Gurumurthy, Rajendra; Kumar, Naveen; Chumduri, Cindrilla.
In: STAR Protocols, Vol. 2, No. 4, 100969, 17.11.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

AU - Kumar Gurumurthy, Rajendra

AU - Kumar, Naveen

AU - Chumduri, Cindrilla

PY - 2021/11/17

Y1 - 2021/11/17

N2 - Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

AB - Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

KW - Gene Expression

KW - In Situ Hybridization

KW - Microscopy

UR - http://www.scopus.com/inward/record.url?scp=85119171293&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2021.100969

DO - 10.1016/j.xpro.2021.100969

M3 - Journal article

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 100969

ER -

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this