Soil invertebrate biodiversity: Next Generation Monitoring, Bioscience Symposium 2018

TY - GEN

T1 - Soil invertebrate biodiversity

T2 - Next Generation Monitoring, Bioscience Symposium 2018

AU - Krogh, P H

AU - Qin, Jiayi

AU - Winding, Anne

AU - Kot, Witold

AU - Ellegaard-Jensen, Lea

AU - Hansen, Lars Hestbjerg

PY - 2018/12/4

Y1 - 2018/12/4

N2 - Detection of biodiversity by molecular techniques is attractive as taxonomic expertise is scarce and time-consuming. The environment contains both live animals and all sorts of traces of DNA from their activities and bodies. Currently we take two approaches to sample soil invertebrate DNA. For earthworms, DNA extracted from soil is feasible, while for the small microarthropods, 0.1 to 2 mm, extraction of live animals from soil and subsequently extraction of DNA from a bulk sample of these intact animals provide undegraded DNA. The soil eDNA methodology is not standardized because a number of steps is not fully tested. E.g., number of soil cores and subsampling of composite samples to arrive at an accurate estimate of species richness, and methodological details of the DNA extraction is not yet optimized. For earthworms, our results indicate that the potential presence of DNA from non-resident species or recent inhabiting species are not a problem. Comparing different soil conditions within the same agricultural habitat due to different agricultural practices can be detected by earthworm eDNA but the response is different from the response seen by the conventional approach. eDNA provides a species list including cryptic species and clones that could not be detected by conventional morphological characters.

AB - Detection of biodiversity by molecular techniques is attractive as taxonomic expertise is scarce and time-consuming. The environment contains both live animals and all sorts of traces of DNA from their activities and bodies. Currently we take two approaches to sample soil invertebrate DNA. For earthworms, DNA extracted from soil is feasible, while for the small microarthropods, 0.1 to 2 mm, extraction of live animals from soil and subsequently extraction of DNA from a bulk sample of these intact animals provide undegraded DNA. The soil eDNA methodology is not standardized because a number of steps is not fully tested. E.g., number of soil cores and subsampling of composite samples to arrive at an accurate estimate of species richness, and methodological details of the DNA extraction is not yet optimized. For earthworms, our results indicate that the potential presence of DNA from non-resident species or recent inhabiting species are not a problem. Comparing different soil conditions within the same agricultural habitat due to different agricultural practices can be detected by earthworm eDNA but the response is different from the response seen by the conventional approach. eDNA provides a species list including cryptic species and clones that could not be detected by conventional morphological characters.

M3 - Other contribution

CY - Moesgaard Museum

ER -