Skin biopsy analysis in diabetic neuropathy

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Skin biopsy analysis in diabetic neuropathy. / Karlsson, Páll.

Diabetic Neuropathy. Elsevier, 2022. p. 79-90.

Research output: Contribution to book/anthology/report/proceedingBook chapterResearchpeer-review

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Karlsson P. Skin biopsy analysis in diabetic neuropathy. In Diabetic Neuropathy. Elsevier. 2022. p. 79-90 doi: 10.1016/B978-0-12-820669-0.00013-X

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Karlsson, Páll. / Skin biopsy analysis in diabetic neuropathy. Diabetic Neuropathy. Elsevier, 2022. pp. 79-90

Bibtex

@inbook{7529fd5d6983406a8d6d59255017df36,
title = "Skin biopsy analysis in diabetic neuropathy",
abstract = "Skin biopsies and estimation of intraepidermal nerve fiber density (IENFD) upon immunohistological staining have proven useful in the diagnosis of diabetic neuropathy (DN) with good diagnostic performance. They can be repeated over time to follow disease progression or to follow efficacy of an intervention. However, the low IENFD observed in patients does not seem to explain why some DN patients develop neuropathic pain while others do not. It is therefore important to extract further information from the biopsies. In addition, immunohistological stainings are difficult to standardize, and processing and staining protocols of skin biopsies are heterogeneous, often resulting in varying results between laboratories. This chapter discusses in detail the advantages and disadvantages of skin biopsies, what they can and cannot do, and gives a detailed protocol on how to take and process the skin biopsies and how to stain the IENFs. Lastly, the chapter provides a number of examples of promising biomarkers that may increase our knowledge of why some patients develop painful DN.",
keywords = "Diabetes, Diabetic neuropathy, IENFD, Neuropathic pain, PGP 9.5, Skin biopsy",
author = "P{\'a}ll Karlsson",
note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Inc. All rights reserved.",
year = "2022",
doi = "10.1016/B978-0-12-820669-0.00013-X",
language = "English",
isbn = "9780128206706",
pages = "79--90",
booktitle = "Diabetic Neuropathy",
publisher = "Elsevier",
address = "Netherlands",

}

RIS

TY - CHAP

T1 - Skin biopsy analysis in diabetic neuropathy

AU - Karlsson, Páll

N1 - Publisher Copyright: © 2022 Elsevier Inc. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Skin biopsies and estimation of intraepidermal nerve fiber density (IENFD) upon immunohistological staining have proven useful in the diagnosis of diabetic neuropathy (DN) with good diagnostic performance. They can be repeated over time to follow disease progression or to follow efficacy of an intervention. However, the low IENFD observed in patients does not seem to explain why some DN patients develop neuropathic pain while others do not. It is therefore important to extract further information from the biopsies. In addition, immunohistological stainings are difficult to standardize, and processing and staining protocols of skin biopsies are heterogeneous, often resulting in varying results between laboratories. This chapter discusses in detail the advantages and disadvantages of skin biopsies, what they can and cannot do, and gives a detailed protocol on how to take and process the skin biopsies and how to stain the IENFs. Lastly, the chapter provides a number of examples of promising biomarkers that may increase our knowledge of why some patients develop painful DN.

AB - Skin biopsies and estimation of intraepidermal nerve fiber density (IENFD) upon immunohistological staining have proven useful in the diagnosis of diabetic neuropathy (DN) with good diagnostic performance. They can be repeated over time to follow disease progression or to follow efficacy of an intervention. However, the low IENFD observed in patients does not seem to explain why some DN patients develop neuropathic pain while others do not. It is therefore important to extract further information from the biopsies. In addition, immunohistological stainings are difficult to standardize, and processing and staining protocols of skin biopsies are heterogeneous, often resulting in varying results between laboratories. This chapter discusses in detail the advantages and disadvantages of skin biopsies, what they can and cannot do, and gives a detailed protocol on how to take and process the skin biopsies and how to stain the IENFs. Lastly, the chapter provides a number of examples of promising biomarkers that may increase our knowledge of why some patients develop painful DN.

KW - Diabetes

KW - Diabetic neuropathy

KW - IENFD

KW - Neuropathic pain

KW - PGP 9.5

KW - Skin biopsy

UR - http://www.scopus.com/inward/record.url?scp=85131274371&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-820669-0.00013-X

DO - 10.1016/B978-0-12-820669-0.00013-X

M3 - Book chapter

AN - SCOPUS:85131274371

SN - 9780128206706

SP - 79

EP - 90

BT - Diabetic Neuropathy

PB - Elsevier

ER -