Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors

Esben Kjær Unmack Larsen; Thomas Nielsen; Thomas Wittenborn; Henrik Birkedal; Thomas Vorup-Jensen; Mogens H Jakobsen; Leif Østergaard; Michael R Horsman; Flemming Besenbacher; Kenneth A Howard; Jørgen Kjems

doi:10.1021/nn900330m

Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors

Esben Kjær Unmack Larsen, Thomas Nielsen, Thomas Wittenborn, Henrik Birkedal, Thomas Vorup-Jensen, Mogens H Jakobsen, Leif Østergaard, Michael R Horsman, Flemming Besenbacher, Kenneth A Howard, Jørgen Kjems

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

239 Citations (Scopus)

Abstract

Magnetic nanoparticles (MNP) can be used as contrast-enhancing agents to visualize tumors by magnetic resonance imaging (MRI). Here we describe an easy synthesis method of magnetic nanoparticles coated with polyethylene glycol (PEG) and demonstrate size-dependent accumulation in murine tumors following intravenous injection. Biocompatible iron oxide MNPs coated with PEG were prepared by replacing oleic acid with a biocompatible and commercially available silane-PEG to provide an easy and effective method for chemical coating. The colloidal stable PEGylated MNPs were magnetically separated into two distinct size subpopulations of 20 and 40 nm mean diameters with increased phagocytic uptake observed for the 40 nm size range in vitro. MRI detection revealed greater iron accumulation in murine tumors for 40 nm nanoparticles after intravenous injection. The enhanced MRI contrast of the larger MNPs in the tumor may be a combined result of the size-dependent extravasation and capture by macrophages in the tumor, providing important considerations for improved bioimaging approaches.

Original language	English
Journal	A C S Nano
Volume	3
Issue	7
Pages (from-to)	1947-51
Number of pages	5
ISSN	1936-0851
DOIs	https://doi.org/10.1021/nn900330m
Publication status	Published - 2009

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10.1021/nn900330m

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@article{17fa1b408b1311deaab2000ea68e967b,

title = "Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors",

abstract = "Magnetic nanoparticles (MNP) can be used as contrast-enhancing agents to visualize tumors by magnetic resonance imaging (MRI). Here we describe an easy synthesis method of magnetic nanoparticles coated with polyethylene glycol (PEG) and demonstrate size-dependent accumulation in murine tumors following intravenous injection. Biocompatible iron oxide MNPs coated with PEG were prepared by replacing oleic acid with a biocompatible and commercially available silane-PEG to provide an easy and effective method for chemical coating. The colloidal stable PEGylated MNPs were magnetically separated into two distinct size subpopulations of 20 and 40 nm mean diameters with increased phagocytic uptake observed for the 40 nm size range in vitro. MRI detection revealed greater iron accumulation in murine tumors for 40 nm nanoparticles after intravenous injection. The enhanced MRI contrast of the larger MNPs in the tumor may be a combined result of the size-dependent extravasation and capture by macrophages in the tumor, providing important considerations for improved bioimaging approaches.",

author = "Larsen, {Esben Kj{\ae}r Unmack} and Thomas Nielsen and Thomas Wittenborn and Henrik Birkedal and Thomas Vorup-Jensen and Jakobsen, {Mogens H} and Leif {\O}stergaard and Horsman, {Michael R} and Flemming Besenbacher and Howard, {Kenneth A} and J{\o}rgen Kjems",

year = "2009",

doi = "10.1021/nn900330m",

language = "English",

volume = "3",

pages = "1947--51",

journal = "A C S Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "7",

}

Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors. / Larsen, Esben Kjær Unmack; Nielsen, Thomas ; Wittenborn, Thomas et al.
In: A C S Nano, Vol. 3, No. 7, 2009, p. 1947-51.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors

AU - Larsen, Esben Kjær Unmack

AU - Nielsen, Thomas

AU - Wittenborn, Thomas

AU - Birkedal, Henrik

AU - Vorup-Jensen, Thomas

AU - Jakobsen, Mogens H

AU - Østergaard, Leif

AU - Horsman, Michael R

AU - Besenbacher, Flemming

AU - Howard, Kenneth A

AU - Kjems, Jørgen

PY - 2009

Y1 - 2009

N2 - Magnetic nanoparticles (MNP) can be used as contrast-enhancing agents to visualize tumors by magnetic resonance imaging (MRI). Here we describe an easy synthesis method of magnetic nanoparticles coated with polyethylene glycol (PEG) and demonstrate size-dependent accumulation in murine tumors following intravenous injection. Biocompatible iron oxide MNPs coated with PEG were prepared by replacing oleic acid with a biocompatible and commercially available silane-PEG to provide an easy and effective method for chemical coating. The colloidal stable PEGylated MNPs were magnetically separated into two distinct size subpopulations of 20 and 40 nm mean diameters with increased phagocytic uptake observed for the 40 nm size range in vitro. MRI detection revealed greater iron accumulation in murine tumors for 40 nm nanoparticles after intravenous injection. The enhanced MRI contrast of the larger MNPs in the tumor may be a combined result of the size-dependent extravasation and capture by macrophages in the tumor, providing important considerations for improved bioimaging approaches.

AB - Magnetic nanoparticles (MNP) can be used as contrast-enhancing agents to visualize tumors by magnetic resonance imaging (MRI). Here we describe an easy synthesis method of magnetic nanoparticles coated with polyethylene glycol (PEG) and demonstrate size-dependent accumulation in murine tumors following intravenous injection. Biocompatible iron oxide MNPs coated with PEG were prepared by replacing oleic acid with a biocompatible and commercially available silane-PEG to provide an easy and effective method for chemical coating. The colloidal stable PEGylated MNPs were magnetically separated into two distinct size subpopulations of 20 and 40 nm mean diameters with increased phagocytic uptake observed for the 40 nm size range in vitro. MRI detection revealed greater iron accumulation in murine tumors for 40 nm nanoparticles after intravenous injection. The enhanced MRI contrast of the larger MNPs in the tumor may be a combined result of the size-dependent extravasation and capture by macrophages in the tumor, providing important considerations for improved bioimaging approaches.

U2 - 10.1021/nn900330m

DO - 10.1021/nn900330m

M3 - Journal article

C2 - 19572620

SN - 1936-0851

VL - 3

SP - 1947

EP - 1951

JO - A C S Nano

JF - A C S Nano

IS - 7

ER -

Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors

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