Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle

Jakob Wang; Emil Rindom; Thomas Groennebaek; Peter Sieljacks; Jesper Emil Jakobsgaard; Jean Farup; Kristian Vissing; Thomas Holm Pedersen; Frank Vincenzo de Paoli

doi:10.1007/s10974-023-09644-6

Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle

Jakob Wang, Emil Rindom, Thomas Groennebaek, Peter Sieljacks, Jesper Emil Jakobsgaard, Jean Farup, Kristian Vissing, Thomas Holm Pedersen, Frank Vincenzo de Paoli^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Contractile function of skeletal muscle relies on the ability of muscle fibers to trigger and propagate action potentials (APs). These electrical signals are created by transmembrane ion transport through ion channels and membrane transporter systems. In this regard, the Cl - ion channel 1 (ClC-1) and the Na +/K --ATPase (NKA) are central for maintaining ion homeostasis across the sarcolemma during intense contractile activity. Therefore, this randomized controlled trial aimed to investigate the changes in ClC-1 and specific NKA subunit isoform expression in response to six weeks (18 training sessions) of high-load resistance exercise (HLRE) and low-load blood flow restricted resistance exercise (BFRRE), respectively. HLRE was conducted as 4 sets of 12 repetitions of knee extensions performed at 70% of 1 repetition maximum (RM), while BFRRE was conducted as 4 sets of knee extensions at 30% of 1RM performed to volitional fatigue. Furthermore, the potential associations between protein expression and contractile performance were investigated. We show that muscle ClC-1 abundance was not affected by either exercise modality, whereas NKA subunit isoforms [Formula: see text] 2 and [Formula: see text] 1 increased equally by appx. 80-90% with BFRRE (p < 0.05) and 70-80% with HLRE (p < 0.05). No differential impact between exercise modalities was observed. At baseline, ClC-1 protein expression correlated inversely with dynamic knee extensor strength (r=-0.365, p = 0.04), whereas no correlation was observed between NKA subunit content and contractile performance at baseline. However, training-induced changes in NKA [Formula: see text] 2 subunit (r = 0.603, p < 0.01) and [Formula: see text] 1 subunit (r = 0.453, p < 0.05) correlated with exercise-induced changes in maximal voluntary contraction. These results suggest that the initial adaptation to resistance-based exercise does not involve changes in ClC-1 abundance in untrained skeletal muscle, and that increased content of NKA subunits may facilitate increases in maximal force production.

Original language	English
Journal	Journal of Muscle Research and Cell Motility
Volume	44
Issue	1
Pages (from-to)	25-36
Number of pages	12
ISSN	0142-4319
DOIs	https://doi.org/10.1007/s10974-023-09644-6
Publication status	Published - Apr 2023

Keywords

Cl conductance
Exercise
Function
Muscle excitability

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10.1007/s10974-023-09644-6

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Wang, J., Rindom, E., Groennebaek, T., Sieljacks, P., Jakobsgaard, J. E., Farup, J., Vissing, K., Pedersen, T. H., & de Paoli, F. V. (2023). Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle. Journal of Muscle Research and Cell Motility, 44(1), 25-36. https://doi.org/10.1007/s10974-023-09644-6

@article{d4608b22632d4bf0b2fa4c3fac743a00,

title = "Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle",

abstract = "Contractile function of skeletal muscle relies on the ability of muscle fibers to trigger and propagate action potentials (APs). These electrical signals are created by transmembrane ion transport through ion channels and membrane transporter systems. In this regard, the Cl - ion channel 1 (ClC-1) and the Na +/K --ATPase (NKA) are central for maintaining ion homeostasis across the sarcolemma during intense contractile activity. Therefore, this randomized controlled trial aimed to investigate the changes in ClC-1 and specific NKA subunit isoform expression in response to six weeks (18 training sessions) of high-load resistance exercise (HLRE) and low-load blood flow restricted resistance exercise (BFRRE), respectively. HLRE was conducted as 4 sets of 12 repetitions of knee extensions performed at 70% of 1 repetition maximum (RM), while BFRRE was conducted as 4 sets of knee extensions at 30% of 1RM performed to volitional fatigue. Furthermore, the potential associations between protein expression and contractile performance were investigated. We show that muscle ClC-1 abundance was not affected by either exercise modality, whereas NKA subunit isoforms [Formula: see text] 2 and [Formula: see text] 1 increased equally by appx. 80-90% with BFRRE (p < 0.05) and 70-80% with HLRE (p < 0.05). No differential impact between exercise modalities was observed. At baseline, ClC-1 protein expression correlated inversely with dynamic knee extensor strength (r=-0.365, p = 0.04), whereas no correlation was observed between NKA subunit content and contractile performance at baseline. However, training-induced changes in NKA [Formula: see text] 2 subunit (r = 0.603, p < 0.01) and [Formula: see text] 1 subunit (r = 0.453, p < 0.05) correlated with exercise-induced changes in maximal voluntary contraction. These results suggest that the initial adaptation to resistance-based exercise does not involve changes in ClC-1 abundance in untrained skeletal muscle, and that increased content of NKA subunits may facilitate increases in maximal force production. ",

keywords = "Cl conductance, Exercise, Function, Muscle excitability",

author = "Jakob Wang and Emil Rindom and Thomas Groennebaek and Peter Sieljacks and Jakobsgaard, {Jesper Emil} and Jean Farup and Kristian Vissing and Pedersen, {Thomas Holm} and {de Paoli}, {Frank Vincenzo}",

year = "2023",

month = apr,

doi = "10.1007/s10974-023-09644-6",

language = "English",

volume = "44",

pages = "25--36",

journal = "Journal of Muscle Research and Cell Motility",

issn = "0142-4319",

publisher = "Springer",

number = "1",

}

Wang, J , Rindom, E, Groennebaek, T, Sieljacks, P, Jakobsgaard, JE, Farup, J , Vissing, K , Pedersen, TH & de Paoli, FV 2023, 'Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle', Journal of Muscle Research and Cell Motility, vol. 44, no. 1, pp. 25-36. https://doi.org/10.1007/s10974-023-09644-6

Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle. / Wang, Jakob ; Rindom, Emil; Groennebaek, Thomas et al.
In: Journal of Muscle Research and Cell Motility, Vol. 44, No. 1, 04.2023, p. 25-36.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle

AU - Wang, Jakob

AU - Rindom, Emil

AU - Groennebaek, Thomas

AU - Sieljacks, Peter

AU - Jakobsgaard, Jesper Emil

AU - Farup, Jean

AU - Vissing, Kristian

AU - Pedersen, Thomas Holm

AU - de Paoli, Frank Vincenzo

PY - 2023/4

Y1 - 2023/4

N2 - Contractile function of skeletal muscle relies on the ability of muscle fibers to trigger and propagate action potentials (APs). These electrical signals are created by transmembrane ion transport through ion channels and membrane transporter systems. In this regard, the Cl - ion channel 1 (ClC-1) and the Na +/K --ATPase (NKA) are central for maintaining ion homeostasis across the sarcolemma during intense contractile activity. Therefore, this randomized controlled trial aimed to investigate the changes in ClC-1 and specific NKA subunit isoform expression in response to six weeks (18 training sessions) of high-load resistance exercise (HLRE) and low-load blood flow restricted resistance exercise (BFRRE), respectively. HLRE was conducted as 4 sets of 12 repetitions of knee extensions performed at 70% of 1 repetition maximum (RM), while BFRRE was conducted as 4 sets of knee extensions at 30% of 1RM performed to volitional fatigue. Furthermore, the potential associations between protein expression and contractile performance were investigated. We show that muscle ClC-1 abundance was not affected by either exercise modality, whereas NKA subunit isoforms [Formula: see text] 2 and [Formula: see text] 1 increased equally by appx. 80-90% with BFRRE (p < 0.05) and 70-80% with HLRE (p < 0.05). No differential impact between exercise modalities was observed. At baseline, ClC-1 protein expression correlated inversely with dynamic knee extensor strength (r=-0.365, p = 0.04), whereas no correlation was observed between NKA subunit content and contractile performance at baseline. However, training-induced changes in NKA [Formula: see text] 2 subunit (r = 0.603, p < 0.01) and [Formula: see text] 1 subunit (r = 0.453, p < 0.05) correlated with exercise-induced changes in maximal voluntary contraction. These results suggest that the initial adaptation to resistance-based exercise does not involve changes in ClC-1 abundance in untrained skeletal muscle, and that increased content of NKA subunits may facilitate increases in maximal force production.

AB - Contractile function of skeletal muscle relies on the ability of muscle fibers to trigger and propagate action potentials (APs). These electrical signals are created by transmembrane ion transport through ion channels and membrane transporter systems. In this regard, the Cl - ion channel 1 (ClC-1) and the Na +/K --ATPase (NKA) are central for maintaining ion homeostasis across the sarcolemma during intense contractile activity. Therefore, this randomized controlled trial aimed to investigate the changes in ClC-1 and specific NKA subunit isoform expression in response to six weeks (18 training sessions) of high-load resistance exercise (HLRE) and low-load blood flow restricted resistance exercise (BFRRE), respectively. HLRE was conducted as 4 sets of 12 repetitions of knee extensions performed at 70% of 1 repetition maximum (RM), while BFRRE was conducted as 4 sets of knee extensions at 30% of 1RM performed to volitional fatigue. Furthermore, the potential associations between protein expression and contractile performance were investigated. We show that muscle ClC-1 abundance was not affected by either exercise modality, whereas NKA subunit isoforms [Formula: see text] 2 and [Formula: see text] 1 increased equally by appx. 80-90% with BFRRE (p < 0.05) and 70-80% with HLRE (p < 0.05). No differential impact between exercise modalities was observed. At baseline, ClC-1 protein expression correlated inversely with dynamic knee extensor strength (r=-0.365, p = 0.04), whereas no correlation was observed between NKA subunit content and contractile performance at baseline. However, training-induced changes in NKA [Formula: see text] 2 subunit (r = 0.603, p < 0.01) and [Formula: see text] 1 subunit (r = 0.453, p < 0.05) correlated with exercise-induced changes in maximal voluntary contraction. These results suggest that the initial adaptation to resistance-based exercise does not involve changes in ClC-1 abundance in untrained skeletal muscle, and that increased content of NKA subunits may facilitate increases in maximal force production.

KW - Cl conductance

KW - Exercise

KW - Function

KW - Muscle excitability

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U2 - 10.1007/s10974-023-09644-6

DO - 10.1007/s10974-023-09644-6

M3 - Journal article

C2 - 37014477

SN - 0142-4319

VL - 44

SP - 25

EP - 36

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

IS - 1

ER -

Wang J , Rindom E, Groennebaek T, Sieljacks P, Jakobsgaard JE, Farup J et al. Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle. Journal of Muscle Research and Cell Motility. 2023 Apr;44(1):25-36. doi: 10.1007/s10974-023-09644-6

Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle

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