siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

Morten Ø Andersen; Jens V Nygaard; Jorge S Burns; Merete K Raarup; Jens R Nyengaard; Cody Bünger; Flemming Besenbacher; Kenneth A Howard; Moustapha Kassem; Jørgen Kjems

doi:10.1038/mt.2010.166

siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

Morten Ø Andersen, Jens V Nygaard, Jorge S Burns, Merete K Raarup, Jens R Nyengaard, Cody Bünger, Flemming Besenbacher, Kenneth A Howard, Moustapha Kassem, Jørgen Kjems

Department of Clinical Medicine - Orthopaedic surgery

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

74 Citations (Scopus)

Abstract

The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.

Original language	English
Journal	Molecular Therapy
Volume	18
Issue	11
Pages (from-to)	2018-2027
Number of pages	10
ISSN	1525-0016
DOIs	https://doi.org/10.1038/mt.2010.166
Publication status	Published - 2010

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10.1038/mt.2010.166

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@article{c585c018c2b64c459a1680e72f435693,

title = "siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells",

abstract = "The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.",

author = "Andersen, {Morten {\O}} and Nygaard, {Jens V} and Burns, {Jorge S} and Raarup, {Merete K} and Nyengaard, {Jens R} and Cody B{\"u}nger and Flemming Besenbacher and Howard, {Kenneth A} and Moustapha Kassem and J{\o}rgen Kjems",

year = "2010",

doi = "10.1038/mt.2010.166",

language = "English",

volume = "18",

pages = "2018--2027",

journal = "Molecular Therapy",

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}

siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells. / Andersen, Morten Ø; Nygaard, Jens V; Burns, Jorge S et al.
In: Molecular Therapy, Vol. 18, No. 11, 2010, p. 2018-2027.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

AU - Andersen, Morten Ø

AU - Nygaard, Jens V

AU - Burns, Jorge S

AU - Raarup, Merete K

AU - Nyengaard, Jens R

AU - Bünger, Cody

AU - Besenbacher, Flemming

AU - Howard, Kenneth A

AU - Kassem, Moustapha

AU - Kjems, Jørgen

PY - 2010

Y1 - 2010

N2 - The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.

AB - The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.

U2 - 10.1038/mt.2010.166

DO - 10.1038/mt.2010.166

M3 - Journal article

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SN - 1525-0016

VL - 18

SP - 2018

EP - 2027

JO - Molecular Therapy

JF - Molecular Therapy

IS - 11

ER -

siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

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