Sinorhizobium fredii HH103 nolR and nodD2 mutants gain capacity for infection thread invasion of Lotus japonicus Gifu and Lotus burttii

Sebastián Acosta-Jurado, Dulce Nombre Rodríguez-Navarro, Yasuyuki Kawaharada, Miguel A. Rodríguez-Carvajal, Antonio Gil-Serrano, María E. Soria-Díaz, Francisco Pérez-Montaño, Juan Fernández-Perea, Yanbo Niu, Cynthia Alias-Villegas, Irene Jiménez-Guerrero, Pilar Navarro-Gómez, Francisco Javier López-Baena, Simon Kelly, Niels Sandal, Jens Stougaard, José E. Ruiz-Sainz, José María Vinardell*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Sinorhizobium fredii HH103 Rif R , a broad-host-range rhizobial strain, forms ineffective nodules with Lotus japonicus but induces nitrogen-fixing nodules in Lotus burttii roots that are infected by intercellular entry. Here we show that HH103 Rif R nolR or nodD2 mutants gain the ability to induce infection thread formation and to form nitrogen-fixing nodules in L. japonicus Gifu. Microscopy studies showed that the mode of infection of L. burttii roots by the nodD2 and nolR mutants switched from intercellular entry to infection threads (ITs). In the presence of the isoflavone genistein, both mutants overproduced Nod-factors. Transcriptomic analyses showed that, in the presence of Lotus japonicus Gifu root exudates, genes related to Nod factors production were overexpressed in both mutants in comparison to HH103 Rif R . Complementation of the nodD2 and nolR mutants provoked a decrease in Nod-factor production, the incapacity to form nitrogen-fixing nodules with L. japonicus Gifu and restored the intercellular way of infection in L. burttii. Thus, the capacity of S. fredii HH103 Rif R nodD2 and nolR mutants to infect L. burttii and L. japonicus Gifu by ITs and fix nitrogen L. japonicus Gifu might be correlated with Nod-factor overproduction, although other bacterial symbiotic signals could also be involved.

Original languageEnglish
JournalEnvironmental Microbiology
Volume21
Issue5
Pages (from-to)1718-1739
Number of pages22
ISSN1462-2912
DOIs
Publication statusPublished - 1 May 2019

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