Singlet Oxygen Sensor Green: Photochemical Behavior in Solution and in a Mammalian Cell

Anita Gollmer; Jacob Arnbjerg; Frances Helen Blaikie; Brian Wett Pedersen; Thomas Breitenbach; Kim Daasbjerg; Marianne Glasius; Peter Remsen Ogilby

Singlet Oxygen Sensor Green: Photochemical Behavior in Solution and in a Mammalian Cell

Anita Gollmer, Jacob Arnbjerg, Frances Helen Blaikie, Brian Wett Pedersen, Thomas Breitenbach, Kim Daasbjerg, Marianne Glasius, Peter Remsen Ogilby

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green® (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.

Original language	English
Journal	Photochemistry and Photobiology
Volume	87
Pages (from-to)	671-679
ISSN	0031-8655
Publication status	Published - 2011

Cite this

@article{257f260acef749da818bc81ff5f960b7,

title = "Singlet Oxygen Sensor Green: Photochemical Behavior in Solution and in a Mammalian Cell",

abstract = "The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green{\textregistered} (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.",

author = "Anita Gollmer and Jacob Arnbjerg and Blaikie, {Frances Helen} and Pedersen, {Brian Wett} and Thomas Breitenbach and Kim Daasbjerg and Marianne Glasius and Ogilby, {Peter Remsen}",

year = "2011",

language = "English",

volume = "87",

pages = "671--679",

journal = "Photochemistry and Photobiology",

issn = "0031-8655",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Singlet Oxygen Sensor Green: Photochemical Behavior in Solution and in a Mammalian Cell

AU - Gollmer, Anita

AU - Arnbjerg, Jacob

AU - Blaikie, Frances Helen

AU - Pedersen, Brian Wett

AU - Breitenbach, Thomas

AU - Daasbjerg, Kim

AU - Glasius, Marianne

AU - Ogilby, Peter Remsen

PY - 2011

Y1 - 2011

N2 - The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green® (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.

AB - The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green® (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.

M3 - Journal article

SN - 0031-8655

VL - 87

SP - 671

EP - 679

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

ER -

Singlet Oxygen Sensor Green: Photochemical Behavior in Solution and in a Mammalian Cell

Abstract

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