Single-step rapid assembly of DNA origami nanostructures for addressable nanoscale bioreactors

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  • Yanming Fu, Chinese Academy of Sciences, China
  • Dongdong Zeng, Chinese Academy of Sciences, China
  • Jie Chao, Chinese Academy of Sciences, China
  • Yanqiu Jin, Chinese Academy of Sciences, China
  • Zhao Zhang, Denmark
  • Huajie Liu
  • ,
  • Di Li, Chinese Academy of Sciences, China
  • Hongwei Ma, Chinese Academy of Sciences, China
  • Qing Huang, Chinese Academy of Sciences, China
  • Kurt Vesterager Gothelf
  • Chunhai Fan, Chinese Academy of Sciences, China
Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based 1D nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments.
Original languageEnglish
JournalJournal of American Chemical Society
Pages (from-to)696-702
Number of pages7
Publication statusPublished - 2013

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