Single molecule microscopy methods for the study of DNA origami structures

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Single molecule microscopy methods for the study of DNA origami structures. / Birkedal, Victoria; Dong, Mingdong; Golas, Monika M; Sander, Bjoern; Andersen, Ebbe Sloth; Gothelf, Kurt Vesterager; Besenbacher, Flemming; Kjems, Jørgen.

In: Microscopy Research and Technique, Vol. 74, No. 7, 07.2011, p. 688-698.

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@article{f8790d4dd39b45d29e0aa32ab7906dd4,
title = "Single molecule microscopy methods for the study of DNA origami structures",
abstract = "Single molecule microscopy techniques play an important role in the investigation of advanced DNA structures such as those created by the DNA origami method. Three single molecule microscopy techniques are particularly interesting for the investigation of complex self-assembled three-dimensional (3D) DNA nanostructures, namely single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo-EM). Here we discuss the strengths of these three techniques and demonstrate how their interplay can yield very important and unique new insights into the structure and conformation of advanced biological nanostructures. The applications of the three single molecule microscopy techniques are illustrated by focusing on a self-assembled DNA origami 3D box nanostructure. Its size and structure were studied by AFM and cryo-EM, while the lid opening, which can be controlled by the addition of oligonucleotide keys, was recorded by F{\"o}rster/fluorescence resonance energy transfer (FRET) spectroscopy.",
keywords = "DNA, Microscopy, Nanostructures",
author = "Victoria Birkedal and Mingdong Dong and Golas, {Monika M} and Bjoern Sander and Andersen, {Ebbe Sloth} and Gothelf, {Kurt Vesterager} and Flemming Besenbacher and J{\o}rgen Kjems",
note = "Copyright {\circledC} 2010 Wiley-Liss, Inc.",
year = "2011",
month = "7",
doi = "10.1002/jemt.20962",
language = "English",
volume = "74",
pages = "688--698",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "JohnWiley & Sons, Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - Single molecule microscopy methods for the study of DNA origami structures

AU - Birkedal, Victoria

AU - Dong, Mingdong

AU - Golas, Monika M

AU - Sander, Bjoern

AU - Andersen, Ebbe Sloth

AU - Gothelf, Kurt Vesterager

AU - Besenbacher, Flemming

AU - Kjems, Jørgen

N1 - Copyright © 2010 Wiley-Liss, Inc.

PY - 2011/7

Y1 - 2011/7

N2 - Single molecule microscopy techniques play an important role in the investigation of advanced DNA structures such as those created by the DNA origami method. Three single molecule microscopy techniques are particularly interesting for the investigation of complex self-assembled three-dimensional (3D) DNA nanostructures, namely single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo-EM). Here we discuss the strengths of these three techniques and demonstrate how their interplay can yield very important and unique new insights into the structure and conformation of advanced biological nanostructures. The applications of the three single molecule microscopy techniques are illustrated by focusing on a self-assembled DNA origami 3D box nanostructure. Its size and structure were studied by AFM and cryo-EM, while the lid opening, which can be controlled by the addition of oligonucleotide keys, was recorded by Förster/fluorescence resonance energy transfer (FRET) spectroscopy.

AB - Single molecule microscopy techniques play an important role in the investigation of advanced DNA structures such as those created by the DNA origami method. Three single molecule microscopy techniques are particularly interesting for the investigation of complex self-assembled three-dimensional (3D) DNA nanostructures, namely single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo-EM). Here we discuss the strengths of these three techniques and demonstrate how their interplay can yield very important and unique new insights into the structure and conformation of advanced biological nanostructures. The applications of the three single molecule microscopy techniques are illustrated by focusing on a self-assembled DNA origami 3D box nanostructure. Its size and structure were studied by AFM and cryo-EM, while the lid opening, which can be controlled by the addition of oligonucleotide keys, was recorded by Förster/fluorescence resonance energy transfer (FRET) spectroscopy.

KW - DNA

KW - Microscopy

KW - Nanostructures

U2 - 10.1002/jemt.20962

DO - 10.1002/jemt.20962

M3 - Journal article

C2 - 21698717

VL - 74

SP - 688

EP - 698

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

SN - 1059-910X

IS - 7

ER -