Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template

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  • Department of Chemistry
  • Interdisciplinary Nanoscience Center
  • Department of Molecular Biology
  • Department of Physics and Astronomy
DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.
Original languageEnglish
JournalA C S Nano
Volume4
Issue12
Pages (from-to)7475-7480
ISSN1936-0851
DOIs
Publication statusPublished - 22 Nov 2010

    Research areas

  • DNA origami, singlet oxygen, AFM, single molecule imaging, photosensitizer

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