Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template

Sarah Wendelboe Helmig, Alexandru Rotaru, Dumitru Arian, Larisa Kovbasyuk, Jacob Arnbjerg, Peter Remsen Ogilby, Jørgen Kjems, Andiry Mokhir, Flemming Besenbacher, Kurt Vesterager Gothelf

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

    51 Citations (Scopus)

    Abstract

    DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.
    Original languageEnglish
    JournalA C S Nano
    Volume4
    Issue12
    Pages (from-to)7475-7480
    ISSN1936-0851
    DOIs
    Publication statusPublished - 22 Nov 2010

    Keywords

    • DNA origami
    • singlet oxygen
    • AFM
    • single molecule imaging
    • photosensitizer

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