Abstract
DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.
Original language | English |
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Journal | A C S Nano |
Volume | 4 |
Issue | 12 |
Pages (from-to) | 7475-7480 |
ISSN | 1936-0851 |
DOIs | |
Publication status | Published - 22 Nov 2010 |
Keywords
- DNA origami
- singlet oxygen
- AFM
- single molecule imaging
- photosensitizer