Single cell enzyme diagnosis on the chip

Sissel Juul Jensen; Charlotte Harmsen; Mette Juul Nielsen; Magnus Stougaard; Kam W Leong; Birgitta R Knudsen; Yi-Ping Ho

doi:10.1109/MEMSYS.2013.6474392

Single cell enzyme diagnosis on the chip

Sissel Juul Jensen
, Charlotte Harmsen
, Mette Juul Nielsen
, Magnus Stougaard
, Kam W Leong
, Birgitta R Knudsen
, Yi-Ping Ho

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Conference article › Research › peer-review

1 Citation (Scopus)

Abstract

Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms

Original language	English
Journal	International Conference on Micro Electro Mechanical Systems
Pages (from-to)	911 - 914
Number of pages	4
ISSN	1084-6999
DOIs	https://doi.org/10.1109/MEMSYS.2013.6474392
Publication status	Published - 20 Jan 2013
Event	Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on - Taipei, Taiwan Duration: 20 Jan 2013 → 24 Jan 2013

Conference

Conference	Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Country/Territory	Taiwan
City	Taipei
Period	20/01/2013 → 24/01/2013

Access to Document

10.1109/MEMSYS.2013.6474392

Library access?

Check availability with the Royal Danish Library

Cite this

@inproceedings{aac8a1ada9924e7db754983fcb267dcf,

title = "Single cell enzyme diagnosis on the chip",

abstract = "Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms",

author = "Jensen, \{Sissel Juul\} and Charlotte Harmsen and Nielsen, \{Mette Juul\} and Magnus Stougaard and Leong, \{Kam W\} and Knudsen, \{Birgitta R\} and Yi-Ping Ho",

year = "2013",

month = jan,

day = "20",

doi = "10.1109/MEMSYS.2013.6474392",

language = "English",

pages = "911 -- 914",

journal = "International Conference on Micro Electro Mechanical Systems",

issn = "1084-6999",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on ; Conference date: 20-01-2013 Through 24-01-2013",

}

TY - GEN

T1 - Single cell enzyme diagnosis on the chip

AU - Jensen, Sissel Juul

AU - Harmsen, Charlotte

AU - Nielsen, Mette Juul

AU - Stougaard, Magnus

AU - Leong, Kam W

AU - Knudsen, Birgitta R

AU - Ho, Yi-Ping

PY - 2013/1/20

Y1 - 2013/1/20

N2 - Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms

AB - Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms

UR - https://www.scopus.com/pages/publications/84875473876

U2 - 10.1109/MEMSYS.2013.6474392

DO - 10.1109/MEMSYS.2013.6474392

M3 - Conference article

AN - SCOPUS:84875473876

SN - 1084-6999

SP - 911

EP - 914

JO - International Conference on Micro Electro Mechanical Systems

JF - International Conference on Micro Electro Mechanical Systems

T2 - Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Y2 - 20 January 2013 through 24 January 2013

ER -

Single cell enzyme diagnosis on the chip

Abstract

Conference

Access to Document

Library access?

Other files and links

Fingerprint

Cite this